Building invitro 3D human multicellular models of high-grade serous ovarian cancer.
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Volume
3
Pagination
101086 - ?
DOI
10.1016/j.xpro.2021.101086
Journal
STAR Protoc
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Three-dimensional (3D), multicellular invitro models provide a useful platform for studying human cancer biology, particularly through deconvolution of the tumor microenvironment, or where animal models do not recapitulate the human condition. Here, we detail a protocol for building human multicellular models made of patient-derived primary cells and malignant cell lines, which recapitulate features of the tumor microenvironment. This protocol is optimized for building 3D models of high-grade serous ovarian cancer omental metastasis but can be adapted for modeling other cancers. For complete details on the use and execution of this profile, please refer to Delaine-Smith et al. (2021) and Malacrida et al. (2021).
Authors
Malacrida, B; Pearce, OMT; Balkwill, FRCollections
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