Changes in brown adipose tissue lipid mediator signatures with aging, obesity, and DHA supplementation in female mice
Volume
35
Publisher
Publisher URL
DOI
10.1096/fj.202002531R
Journal
FASEB JOURNAL
Issue
ISSN
0892-6638
Metadata
Show full item recordAbstract
Brown adipose tissue (BAT) dysfunction in aging and obesity has been related to chronic unresolved inflammation, which could be mediated by an impaired pro-duction of specialized proresolving lipid mediators (SPMs), such as Lipoxins- LXs, Resolvins- Rvs, Protectins- PDs, and Maresins- MaRs. Our aim was to characterize the changes in BAT SPMs signatures and their association with BAT dysfunction during aging, especially under obesogenic conditions, and their modulation by a docosahex-aenoic acid (DHA)- rich diet. Lipidomic, functional, and molecular studies were per-formed in BAT of 2- and 18- month- old lean (CT) female mice and in 18- month- old diet- induced obese (DIO) mice fed with a high- fat diet (HFD), or a DHA- enriched HFD. Aging downregulated Prdm16 and UCP1 levels, especially in DIO mice, while DHA partially restored them. Arachidonic acid (AA)- derived LXs and DHA- derived MaRs and PDs were the most abundant SPMs in BAT of young CT mice.