Changes in brown adipose tissue lipid mediator signatures with aging, obesity, and DHA supplementation in female mice

Abstract

Brown adipose tissue (BAT) dysfunction in aging and obesity has been related to chronic unresolved inflammation, which could be mediated by an impaired pro-duction of specialized proresolving lipid mediators (SPMs), such as Lipoxins- LXs, Resolvins- Rvs, Protectins- PDs, and Maresins- MaRs. Our aim was to characterize the changes in BAT SPMs signatures and their association with BAT dysfunction during aging, especially under obesogenic conditions, and their modulation by a docosahex-aenoic acid (DHA)- rich diet. Lipidomic, functional, and molecular studies were per-formed in BAT of 2- and 18- month- old lean (CT) female mice and in 18- month- old diet- induced obese (DIO) mice fed with a high- fat diet (HFD), or a DHA- enriched HFD. Aging downregulated Prdm16 and UCP1 levels, especially in DIO mice, while DHA partially restored them. Arachidonic acid (AA)- derived LXs and DHA- derived MaRs and PDs were the most abundant SPMs in BAT of young CT mice.