Lipid Mediator Metabolomics Via LC-MS/MS Profiling and Analysis.
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Series
Methods in Molecular Biology;1730
Volume
1730
Pagination
59 - 72
DOI
10.1007/978-1-4939-7592-1_4
Journal
Methods Mol Biol
Metadata
Show full item recordAbstract
Solid-phase extraction coupled with liquid chromatography tandem mass spectrometry provides a robust and sensitive approach for the identification and quantitation of specialized pro-resolving mediators (lipoxins, resolvins, protectins, and maresins), their pathway markers and the classic eicosanoids. Here, we provide a detailed description of the methodologies employed for the extraction of these mediators from biological systems, setup of the instrumentation, sample processing, and then the procedures followed for their identification and quantitation.