dc.contributor.author | Dalli, J | en_US |
dc.contributor.author | Colas, RA | en_US |
dc.contributor.author | Walker, ME | en_US |
dc.contributor.author | Serhan, CN | en_US |
dc.date.accessioned | 2019-04-01T14:54:53Z | |
dc.date.available | 2017-12-20 | en_US |
dc.date.issued | 2018 | en_US |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/56628 | |
dc.description | protocol | en_US |
dc.description.abstract | Solid-phase extraction coupled with liquid chromatography tandem mass spectrometry provides a robust and sensitive approach for the identification and quantitation of specialized pro-resolving mediators (lipoxins, resolvins, protectins, and maresins), their pathway markers and the classic eicosanoids. Here, we provide a detailed description of the methodologies employed for the extraction of these mediators from biological systems, setup of the instrumentation, sample processing, and then the procedures followed for their identification and quantitation. | en_US |
dc.format.extent | 59 - 72 | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Methods Mol Biol | en_US |
dc.relation.ispartofseries | Methods in Molecular Biology;1730 | |
dc.rights | “This is a post-peer-review, pre-copyedit version of a protocol published in Methods in Molecular Biology. The final publication is available at link.springer.com: https://doi.org/10.1007/978-1-4939-7592-1_4” | |
dc.subject | Eicosanoids | en_US |
dc.subject | Flux analysis | en_US |
dc.subject | Lipid mediator metabololipidomics | en_US |
dc.subject | Liquid chromatography-tandem mass spectrometry | en_US |
dc.subject | Maresin | en_US |
dc.subject | Profiling | en_US |
dc.subject | Protectin | en_US |
dc.subject | Resolvin | en_US |
dc.subject | Chromatography, Liquid | en_US |
dc.subject | Docosahexaenoic Acids | en_US |
dc.subject | Eicosapentaenoic Acid | en_US |
dc.subject | Humans | en_US |
dc.subject | Lipids | en_US |
dc.subject | Lipoxins | en_US |
dc.subject | Metabolomics | en_US |
dc.subject | Solid Phase Extraction | en_US |
dc.subject | Tandem Mass Spectrometry | en_US |
dc.title | Lipid Mediator Metabolomics Via LC-MS/MS Profiling and Analysis. | en_US |
dc.type | Article | |
dc.identifier.doi | 10.1007/978-1-4939-7592-1_4 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/29363065 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 1730 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |
qmul.funder | Sir Henry Dale Fellowship::Wellcome Trust | en_US |