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Derived myeloid lineage induced pluripotent stem as a platform to study human C-C chemokine receptor type 5Δ32 homozygotes.

dc.contributor.authorChen, G
dc.contributor.authorCalcaterra, F
dc.contributor.authorMa, Y
dc.contributor.authorPing, X
dc.contributor.authorPontarini, E
dc.contributor.authorYang, D
dc.contributor.authorOriolo, F
dc.contributor.authorYu, Z
dc.contributor.authorCancellara, A
dc.contributor.authorMikulak, J
dc.contributor.authorHuang, Y
dc.contributor.authorDella Bella, S
dc.contributor.authorLiu, Y
dc.contributor.authorBiesecker, LG
dc.contributor.authorHarper, RL
dc.contributor.authorDalgard, CL
dc.contributor.authorBoehm, M
dc.contributor.authorMavilio, D
dc.date.accessioned2024-06-20T14:10:28Z
dc.date.available2023-10-22
dc.date.available2024-06-20T14:10:28Z
dc.date.issued2023-10-26
dc.identifier.citationTY - JOUR T1 - Derived myeloid lineage induced pluripotent stem as a platform to study human C-C chemokine receptor type 5Δ32 homozygotes AU - Chen, Guibin AU - Calcaterra, Francesca AU - Ma, Yuchi AU - Ping, Xianfeng AU - Pontarini, Elena AU - Yang, Dan AU - Oriolo, Ferdinando AU - Yu, Zhen AU - Cancellara, Assunta AU - Mikulak, Joanna AU - Huang, Yuting AU - Della Bella, Silvia AU - Liu, Yangtengyu AU - Biesecker, Leslie G. AU - Harper, Rebecca L. AU - Dalgard, Clifton L. AU - Boehm, Manfred AU - Mavilio, Domenico Y1 - 2023/11/17 PY - 2023 N1 - doi: 10.1016/j.isci.2023.108331 DO - 10.1016/j.isci.2023.108331 T2 - iScience JF - iScience VL - 26 IS - 11 PB - Elsevier SN - 2589-0042 M3 - doi: 10.1016/j.isci.2023.108331 UR - https://doi.org/10.1016/j.isci.2023.108331 Y2 - 2024/06/20 ER -en_US
dc.identifier.urihttps://qmro.qmul.ac.uk/xmlui/handle/123456789/97566
dc.description.abstractThe C-C chemokine receptor type 5 (CCR5) expressed on immune cells supports inflammatory responses by directing cells to the inflammation site. CCR5 is also a major coreceptor for macrophage tropic human immunodeficiency viruses (R5-HIV-1) and its variants can confer protection from HIV infection, making it an ideal candidate to target for therapy. We developed a stepwise protocol that differentiates induced pluripotent stem cells (iPSCs) from individuals homozygous for the CCR5Δ32 variant and healthy volunteers into myeloid lineage induced monocytes (iMono) and macrophages (iMac). By characterizing iMono and iMac against their primary counterparts, we demonstrated that CCR5Δ32 homozygous cells are endowed with similar pluripotent potential for self-renewal and differentiation as iPSC lines generated from non-variant individuals while also showing resistance to HIV infection. In conclusion, these cells are a platform to investigate CCR5 pathophysiology in HIV-positive and negative individuals and to help develop novel therapies.en_US
dc.format.extent108331 - ?
dc.languageeng
dc.publisherElsevieren_US
dc.relation.ispartofiScience
dc.rightsThis is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.subjectImmunologyen_US
dc.subjectMolecular biologyen_US
dc.subjectStem cells researchen_US
dc.titleDerived myeloid lineage induced pluripotent stem as a platform to study human C-C chemokine receptor type 5Δ32 homozygotes.en_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.isci.2023.108331
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/38026202en_US
pubs.issue11en_US
pubs.notesNot knownen_US
pubs.publication-statusPublished onlineen_US
pubs.volume26en_US
dcterms.dateAccepted2023-10-22
rioxxterms.funderDefault funderen_US
rioxxterms.identifier.projectDefault projecten_US


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