dc.contributor.author | Pitsillidou, C | en_US |
dc.contributor.author | Muradore, I | en_US |
dc.contributor.author | Pontarini, E | en_US |
dc.contributor.author | Bertolotti, M | en_US |
dc.contributor.author | Roberto, A | en_US |
dc.date.accessioned | 2024-06-20T09:01:54Z | |
dc.date.available | 2023-09-20 | en_US |
dc.date.issued | 2023-12-15 | en_US |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/97548 | |
dc.description.abstract | Cellular redox state determinants are traditionally studied using fluorescent microscopy and immunoblot analysis; however, no procedure has been developed for simultaneous measurement in various immune cell subsets. Here, we present a flow cytometry assay for measuring antioxidant defense systems and reactive oxygen species simultaneously in T, B, and natural killer lymphocytes. We describe steps for preparing and treating peripheral blood mononuclear cells, surface and dye staining, cell fixation/permeabilization, and intracellular staining. We then detail machine standardization, acquisition, and analysis. | en_US |
dc.format.extent | 102632 - ? | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | STAR Protoc | en_US |
dc.rights | This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | |
dc.subject | Cell Biology | en_US |
dc.subject | Flow Cytometry | en_US |
dc.subject | Immunology | en_US |
dc.subject | Humans | en_US |
dc.subject | Leukocytes, Mononuclear | en_US |
dc.subject | Flow Cytometry | en_US |
dc.subject | Reactive Oxygen Species | en_US |
dc.subject | Immunoblotting | en_US |
dc.subject | Oxidation-Reduction | en_US |
dc.title | A redox-based characterization of human immune cell subsets by polychromatic flow cytometry. | en_US |
dc.type | Article | |
dc.rights.holder | © 2023 The Authors | |
dc.identifier.doi | 10.1016/j.xpro.2023.102632 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/37838948 | en_US |
pubs.issue | 4 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 4 | en_US |
dcterms.dateAccepted | 2023-09-20 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |