dc.contributor.author | Kennedy, A | en_US |
dc.contributor.author | Griffin, G | en_US |
dc.contributor.author | Freemont, PS | en_US |
dc.contributor.author | Polizzi, KM | en_US |
dc.contributor.author | Moore, SJ | en_US |
dc.date.accessioned | 2024-02-23T09:14:15Z | |
dc.date.available | 2022-08-12 | en_US |
dc.date.issued | 2022 | en_US |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/94831 | |
dc.description.abstract | In synthetic biology, biosensors are routinely coupled with a gene expression system for detecting small molecules and physical signals. We reveal a fluorescent complex, based on the interaction of an Escherichia coli double bond reductase (EcCurA), as a detection unit with its substrate curcumin-we call this a direct protein (DiPro) biosensor. Using a cell-free synthetic biology approach, we use the EcCurA DiPro biosensor to fine tune 10 reaction parameters (cofactor, substrate, and enzyme levels) for cell-free curcumin biosynthesis, assisted through acoustic liquid handling robotics. Overall, we increase EcCurA-curcumin DiPro fluorescence within cell-free reactions by 78-fold. This finding adds to the growing family of protein-ligand complexes that are naturally fluorescent and potentially exploitable for a range of applications, including medical imaging to engineering high-value chemicals. | en_US |
dc.format.extent | 62 - 68 | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Eng Biol | en_US |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. | |
dc.subject | biosensor | en_US |
dc.subject | cell‐free | en_US |
dc.subject | curcumin | en_US |
dc.subject | fine chemical | en_US |
dc.subject | synthetic biology | en_US |
dc.title | A curcumin direct protein biosensor for cell-free prototyping. | en_US |
dc.type | Article | |
dc.rights.holder | © 2022 The Authors. Engineering Biology published by John Wiley & Sons Ltd on behalf of The Institution of Engineering and Technology. | |
dc.identifier.doi | 10.1049/enb2.12024 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/36969103 | en_US |
pubs.issue | 2-3 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published online | en_US |
pubs.volume | 6 | en_US |
dcterms.dateAccepted | 2022-08-12 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |