dc.contributor.author | Ponnusamy, V | |
dc.contributor.author | Ip, RTH | |
dc.contributor.author | Mohamed, MAEK | |
dc.contributor.author | Clarke, P | |
dc.contributor.author | Wozniak, E | |
dc.contributor.author | Mein, C | |
dc.contributor.author | Schwendimann, L | |
dc.contributor.author | Barlas, A | |
dc.contributor.author | Chisholm, P | |
dc.contributor.author | Chakkarapani, E | |
dc.contributor.author | Michael-Titus, AT | |
dc.contributor.author | Gressens, P | |
dc.contributor.author | Yip, PK | |
dc.contributor.author | Shah, DK | |
dc.date.accessioned | 2021-11-03T11:38:30Z | |
dc.date.available | 2021-09-09 | |
dc.date.available | 2021-11-03T11:38:30Z | |
dc.date.issued | 2021-09-30 | |
dc.identifier.citation | Ponnusamy, V., Ip, R.T.H., Mohamed, M.A.E.K. et al. Neuronal let-7b-5p acts through the Hippo-YAP pathway in neonatal encephalopathy. Commun Biol 4, 1143 (2021). https://doi.org/10.1038/s42003-021-02672-3 | en_US |
dc.identifier.other | ARTN 1143 | |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/74987 | |
dc.description.abstract | Despite increasing knowledge on microRNAs, their role in the pathogenesis of neonatal
encephalopathy remains to be elucidated. Herein, we identify let-7b-5p as a significant
microRNA in neonates with moderate to severe encephalopathy from dried blood spots using
next generation sequencing. Validation studies using Reverse Transcription and quantitative
Polymerase Chain Reaction on 45 neonates showed that let-7b-5p expression was increased
on day 1 in neonates with moderate to severe encephalopathy with unfavourable outcome
when compared to those with mild encephalopathy. Mechanistic studies performed on glucose deprived cell cultures and the cerebral cortex of two animal models of perinatal brain
injury, namely hypoxic-ischaemic and intrauterine inflammation models confirm that let-7b5p is associated with the apoptotic Hippo pathway. Significant reduction in neuronal let-7b5p expression corresponded with activated Hippo pathway, with increased neuronal/nuclear
ratio of Yes Associated Protein (YAP) and increased neuronal cleaved caspase-3 expression
in both animal models. Similar results were noted for let-7b-5p and YAP expression in
glucose-deprived cell cultures. Reduced nuclear YAP with decreased intracellular let-7b-5p
correlated with neuronal apoptosis in conditions of metabolic stress. This finding of the
Hippo-YAP association with let-7b needs validation in larger cohorts to further our knowledge on let-7b-5p as a biomarker for neonatal encephalopathy. | en_US |
dc.publisher | Nature Research | en_US |
dc.relation.ispartof | COMMUNICATIONS BIOLOGY | |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/. | |
dc.title | Neuronal let-7b-5p acts through the Hippo-YAP pathway in neonatal encephalopathy | en_US |
dc.type | Article | en_US |
dc.rights.holder | © The Author(s) 2021 | |
dc.identifier.doi | 10.1038/s42003-021-02672-3 | |
pubs.author-url | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000702487200004&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=612ae0d773dcbdba3046f6df545e9f6a | en_US |
pubs.issue | 1 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.publisher-url | https://doi.org/10.1038/s42003-021-02672-3 | |
pubs.volume | 4 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |