The SET1 Complex Selects Actively Transcribed Target Genes via Multivalent Interaction with CpG Island Chromatin
View/ Open
Volume
20
Pagination
2313 - 2327
Publisher
Publisher URL
DOI
10.1016/j.celrep.2017.08.030
Journal
CELL REPORTS
Issue
ISSN
2211-1247
Metadata
Show full item recordAbstract
Chromatin modifications and the promoter-associated epigenome are important for the regulation of gene expression. However, the mechanisms by which chromatin-modifying complexes are targeted to the appropriate gene promoters in vertebrates and how they influence gene expression have remained poorly defined. Here, using a combination of live-cell imaging and functional genomics, we discover that the vertebrate SET1 complex is targeted to actively transcribed gene promoters through CFP1, which engages in a form of multivalent chromatin reading that involves recognition of non-methylated DNA and histone H3 lysine 4 trimethylation (H3K4me3). CFP1 defines SET1 complex occupancy on chromatin, and its multivalent interactions are required for the SET1 complex to place H3K4me3. In the absence of CFP1, gene expression is perturbed, suggesting that normal targeting and function of the SET1 complex are central to creating an appropriately functioning vertebrate promoter-associated epigenome.
Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.
Authors
Brown, DA; Di Cerbo, V; Feldmann, A; Ahn, J; Ito, S; Blackledge, NP; Nakayama, M; McClellan, M; Dimitrova, E; Turberfield, AHCollections
- Centre for Immunobiology [1121]
Licence information
Copyright statements
The following license files are associated with this item: