Browsing Centre for Biochemical Pharmacology by Author "Gould, D"
Now showing items 1-9 of 9
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Assembly PCR synthesis of optimally designed, compact, multi-responsive promoters suited to gene therapy application
Mohamed, H; Chernajovsky, Y; Gould, D (2016-07-08) -
Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay.
Yousaf, N; Gould, D (2017)Confirming the binding of a transcription factor with a particular DNA sequence may be important in characterizing interactions with a synthetic promoter. Electrophoretic mobility shift assay is a powerful approach to ... -
Effects of APC De-Targeting and GAr Modification on the Duration of Luciferase Expression from Plasmid DNA Delivered to Skeletal Muscle
Subang, MC; Fatah, R; Wu, Y; Hannaman, D; Rice, J; Evans, CF; Chernajovsky, Y; Gould, D (2015) -
Gene therapy with an improved doxycycline-regulated plasmid encoding a tumour necrosis factor-alpha inhibitor in experimental arthritis
Gould, D; Yousaf, N; Fatah, R; Subang, MC; Chernajovsky, Y (2007) -
Initial Considerations Before Designing a Promoter Construct.
Gould, D (2017)Before designing a synthetic promoter, it can be helpful to think about its final application. Is the study purely an in vitro exercise in monitoring short-term promoter activity from an episomal vector, or does the promoter ... -
A novel hybrid promoter responsive to pathophysiological and pharmacological regulation
Subang, MC; Fatah, R; Bright, C; Blanco, P; Berenstein, M; Wu, Y; Podhajcer, OL; Winyard, PG; Chernajovsky, Y; Gould, D (2012-04) -
PCR Assembly of Synthetic Promoters.
Mohamed, H; Gould, D (2017)In this chapter, we describe a two-step assembly PCR method to construct synthetic promoters. Essentially, this method takes advantage of specific annealing between complimentary DNA sequences to build random TFBS combinations ... -
The Tetracycline Responsive System.
Yousaf, N; Gould, D (2017)Constitutive gene expression is not always the appropriate expression system because the unphysiological levels of expressed protein could be detrimental in studies examining biological roles of proteins, or continued ... -
Toward gene therapy in rheumatoid arthritis
Young, E; Gould, D; Hart, S (Informa UK Limited, 2020)Introduction: Rheumatoid arthritis (RA) is an autoimmune disease of the joint, affecting 0.24% of the global population. Many patients only respond partially or not at all to current therapies while the systemic complications ...