Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay.
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Accepted version
Embargoed until: 5555-01-01
Reason: Book chapter. Archiving not permitted.
Embargoed until: 5555-01-01
Reason: Book chapter. Archiving not permitted.
Volume
1651
Pagination
11 - 21
DOI
10.1007/978-1-4939-7223-4_2
Metadata
Show full item recordAbstract
Confirming the binding of a transcription factor with a particular DNA sequence may be important in characterizing interactions with a synthetic promoter. Electrophoretic mobility shift assay is a powerful approach to demonstrate the specific DNA sequence that is bound by a transcription factor and also to confirm the specific transcription factor involved in the interaction. In this chapter we describe a method we have successfully used to demonstrate interactions of endogenous transcription factors with sequences derived from endogenous and synthetic promoters.
Authors
Yousaf, N; Gould, DCollections
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