Browsing Centre for Experimental Medicine and Rheumatology (EMR) by Subject "Flow Cytometry"
Now showing items 1-7 of 7
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Association of Defective Regulation of Autoreactive Interleukin-6-Producing Transitional B Lymphocytes With Disease in Patients With Systemic Sclerosis.
(2018-03)OBJECTIVE: Systemic sclerosis (SSc) has the highest case-specific mortality of any rheumatic disease, and no effective therapy is available. A clear manifestation of SSc is the presence of autoantibodies. However, the ... -
High pathogenic potential of low-affinity autoantibodies in experimental autoimmune hemolytic anemia.
(1999-12-06)To assess the potency of low-affinity anti-red blood cell (RBC) autoantibodies in the induction of anemia, we generated an immunoglobulin (Ig)G2a class-switch variant of a 4C8 IgM anti-mouse RBC autoantibody, and compared ... -
Increased frequency of circulating IL-21 producing Th-cells in patients with granulomatosis with polyangiitis (GPA).
(2013)INTRODUCTION: The present study aimed to explore a possible role for IL-21 producing Th-cells in the immunopathogenesis of granulomatosis with polyangiitis (GPA). METHODS: Peripheral blood from 42 GPA patients in remission ... -
Interleukin-4 protects against a genetically linked lupus-like autoimmune syndrome.
(1997-01-06)Interleukin-4 (IL-4) provides support for humoral immune responses through upregulation of T helper (Th) type 2 cell differentiation, but it is not known whether IL-4 promotes antibody-mediated autoimmune diseases such as ... -
Isolation and Characterization of Mouse and Human Follicular Dendritic Cells.
(2017)Follicular dendritic cells (FDCs) reside in the B cell follicles of secondary and tertiary lymphoid tissues where they play key roles in the development and maintenance of lymphoid tissue architecture and function. FDCs ... -
Neutrophil Microvesicles from Healthy Control and Rheumatoid Arthritis Patients Prevent the Inflammatory Activation of Macrophages.
(2018-03)Microvesicles (MVs) are emerging as a novel means to enact cell-to-cell communication in inflammation. Here, we aimed to ascertain the ability of neutrophil-derived MVs to modulate target cell behaviour, the focus being ... -
A redox-based characterization of human immune cell subsets by polychromatic flow cytometry.
(2023-12-15)Cellular redox state determinants are traditionally studied using fluorescent microscopy and immunoblot analysis; however, no procedure has been developed for simultaneous measurement in various immune cell subsets. Here, ...