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Demonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay.

dc.contributor.authorYousaf, Nen_US
dc.contributor.authorGould, Den_US
dc.date.accessioned2017-10-09T15:04:01Z
dc.date.issued2017en_US
dc.date.submitted2017-09-01T14:26:58.264Z
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/27283
dc.description.abstractConfirming the binding of a transcription factor with a particular DNA sequence may be important in characterizing interactions with a synthetic promoter. Electrophoretic mobility shift assay is a powerful approach to demonstrate the specific DNA sequence that is bound by a transcription factor and also to confirm the specific transcription factor involved in the interaction. In this chapter we describe a method we have successfully used to demonstrate interactions of endogenous transcription factors with sequences derived from endogenous and synthetic promoters.en_US
dc.format.extent11 - 21en_US
dc.language.isoenen_US
dc.subjectEMSAen_US
dc.subjectElectrophoretic mobility shift assayen_US
dc.subjectSupershiften_US
dc.subjectTranscription factoren_US
dc.subjectTranscription factor binding siteen_US
dc.subjectBase Sequenceen_US
dc.subjectBinding Sitesen_US
dc.subjectCell Lineen_US
dc.subjectDNAen_US
dc.subjectElectrophoretic Mobility Shift Assayen_US
dc.subjectErythroid-Specific DNA-Binding Factorsen_US
dc.subjectHumansen_US
dc.subjectJurkat Cellsen_US
dc.subjectNF-kappa Ben_US
dc.subjectPromoter Regions, Geneticen_US
dc.subjectProtein Bindingen_US
dc.subjectTranscription Factorsen_US
dc.titleDemonstrating Interactions of Transcription Factors with DNA by Electrophoretic Mobility Shift Assay.en_US
dc.typeBook chapter
dc.identifier.doi10.1007/978-1-4939-7223-4_2en_US
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/28801896en_US
pubs.notesNot knownen_US
pubs.notesSubmitted chapteren_US
pubs.publication-statusPublisheden_US
pubs.volume1651en_US


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