dc.contributor.author | Quent, V | en_US |
dc.contributor.author | Taubenberger, AV | en_US |
dc.contributor.author | Reichert, JC | en_US |
dc.contributor.author | Martine, LC | en_US |
dc.contributor.author | Clements, JA | en_US |
dc.contributor.author | Hutmacher, DW | en_US |
dc.contributor.author | Loessner, D | en_US |
dc.date.accessioned | 2017-08-11T16:36:40Z | |
dc.date.available | 2017-07-11 | en_US |
dc.date.issued | 2018-02 | en_US |
dc.date.submitted | 2017-08-04T09:45:47.436Z | |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/25189 | |
dc.description.abstract | Bone metastases frequently occur in the advanced stages of breast cancer. At this stage, the disease is deemed incurable. To date, the mechanisms of breast cancer-related metastasis to bone are poorly understood. This may be attributed to the lack of appropriate animal models to investigate the complex cancer cell-bone interactions. In this study, two established tissue-engineered bone constructs (TEBCs) were applied to a breast cancer-related metastasis model. A cylindrical medical-grade polycaprolactone-tricalcium phosphate scaffold produced by fused deposition modelling (scaffold 1) was compared with a tubular calcium phosphate-coated polycaprolactone scaffold fabricated by solution electrospinning (scaffold 2) for their potential to generate ectopic humanised bone in NOD/SCID mice. While scaffold 1 was found not suitable to generate a sufficient amount of ectopic bone tissue due to poor ectopic integration, scaffold 2 showed excellent integration into the host tissue, leading to bone formation. To mimic breast cancer cell colonisation to the bone, MDA-MB-231, SUM1315, and MDA-MB-231BO breast cancer cells were cultured in polyethylene glycol-based hydrogels and implanted adjacent to the TEBCs. Histological analysis indicated that the breast cancer cells induced an osteoclastic reaction in the TEBCs, demonstrating analogies to breast cancer-related bone metastasis seen in patients. | en_US |
dc.description.sponsorship | Queensland University of Technology, the Australian Research Council (ARC) and the German Academic Exchange Service (DAAD) | en_US |
dc.format.extent | 494 - 504 | en_US |
dc.language | eng | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | J Tissue Eng Regen Med | en_US |
dc.subject | bone colonisation | en_US |
dc.subject | bone tissue engineering | en_US |
dc.subject | breast cancer | en_US |
dc.subject | fused deposition modelling | en_US |
dc.subject | humanised animal model | en_US |
dc.subject | polycaprolactone scaffolds | en_US |
dc.subject | solution electrospinning | en_US |
dc.title | A humanised tissue-engineered bone model allows species-specific breast cancer-related bone metastasis in vivo. | en_US |
dc.type | Article | |
dc.rights.holder | "This is the peer reviewed version of the following article: Quent V. M. C., Taubenberger A. V., Reichert J. C., Martine L. C., Clements J. A., Hutmacher D. W., and Loessner D. (2017) A humanised tissue-engineered bone model allows species-specific breast cancer-related bone metastasis in vivo, J Tissue Eng Regen Med. which has been published in final form at https://doi.org/10.1002/term.2517 . This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving." | |
dc.identifier.doi | 10.1002/term.2517 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/28714574 | en_US |
pubs.issue | 2 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 12 | en_US |
dcterms.dateAccepted | 2017-07-11 | en_US |