Intracellular autofluorescence: a biomarker for epithelial cancer stem cells.
1161 - 1169
MetadataShow full item record
Cancer stem cells (CSCs) are thought to drive tumor growth, metastasis and chemoresistance. Although surface markers such as CD133 and CD44 have been successfully used to isolate CSCs, their expression is not exclusively linked to the CSC phenotype and is prone to environmental alteration. We identified cells with an autofluorescent subcellular compartment that exclusively showed CSC features across different human tumor types. Primary tumor-derived autofluorescent cells did not overlap with side-population (SP) cells, were enriched in sphere culture and during chemotherapy, strongly expressed pluripotency-associated genes, were highly metastatic and showed long-term in vivo tumorigenicity, even at the single-cell level. Autofluorescence was due to riboflavin accumulation in membrane-bounded cytoplasmic structures bearing ATP-dependent ABCG2 transporters. In summary, we identified and characterized an intrinsic autofluorescent phenotype in CSCs of diverse epithelial cancers and used this marker to isolate and characterize these cells.
AuthorsMiranda-Lorenzo, I; Dorado, J; Lonardo, E; Alcala, S; Serrano, AG; Clausell-Tormos, J; Cioffi, M; Megias, D; Zagorac, S; Balic, A
Showing items related by title, author, creator and subject.
Acromegaly associated with GIST, non-small cell lung carcinoma, clear cell renal carcinoma, multiple myeloma, medulla oblongata tumour, adrenal adenoma, and follicular thyroid nodules. Jawiarczyk-Przybyłowska, A; Wojtczak, B; Whitworth, J; Sutkowski, K; Bidlingmaier, M; Korbonits, M; Bolanowski, M (2019)Acromegaly is associated with increased growth hormone (GH) and insulin-like growth factor-I (IGF-I) secretion which may support tumour development and growth. A 68-year-old woman was diagnosed with acromegaly due to typical ...
Interventions to improve exercise behaviour in sedentary people living with and beyond cancer: a systematic review. Bourke, L; Homer, KE; Thaha, MA; Steed, L; Rosario, DJ; Robb, KA; Saxton, JM; Taylor, SJ (2014-02-18)To systematically review the effects of interventions to improve exercise behaviour in sedentary people living with and beyond cancer.
Tufton, N; Roncaroli, F; Hadjidemetriou, I; Dang, MN; Dénes, J; Guasti, L; Thom, M; Powell, M; Baldeweg, SE; Fersht, N (2017-12)We present the first case of pituitary carcinoma occurring in a patient with a succinate dehydrogenase subunit B (SDHB) mutation and history of paraganglioma. She was initially treated for a glomus tumour with external ...