dc.contributor.author | Haseeb, F | en_US |
dc.contributor.author | Bourdakos, KN | en_US |
dc.contributor.author | Forsyth, E | en_US |
dc.contributor.author | Setchfield, K | en_US |
dc.contributor.author | Gorman, A | en_US |
dc.contributor.author | Venkateswaran, S | en_US |
dc.contributor.author | Wright, AJ | en_US |
dc.contributor.author | Mahajan, S | en_US |
dc.contributor.author | Bradley, M | en_US |
dc.date.accessioned | 2024-04-04T12:37:13Z | |
dc.date.available | 2023-08-28 | en_US |
dc.date.issued | 2023-12 | en_US |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/95922 | |
dc.description.abstract | SIGNIFICANCE: Rapid advances in medical imaging technology, particularly the development of optical systems with non-linear imaging modalities, are boosting deep tissue imaging. The development of reliable standards and phantoms is critical for validation and optimization of these cutting-edge imaging techniques. AIM: We aim to design and fabricate flexible, multi-layered hydrogel-based optical standards and evaluate advanced optical imaging techniques at depth. APPROACH: Standards were made using a robust double-network hydrogel matrix consisting of agarose and polyacrylamide. The materials generated ranged from single layers to more complex constructs consisting of up to seven layers, with modality-specific markers embedded between the layers. RESULTS: These standards proved useful in the determination of the axial scaling factor for light microscopy and allowed for depth evaluation for different imaging modalities (conventional one-photon excitation fluorescence imaging, two-photon excitation fluorescence imaging, second harmonic generation imaging, and coherent anti-Stokes Raman scattering) achieving actual depths of 1550, 1550, 1240, and 1240 μm, respectively. Once fabricated, the phantoms were found to be stable for many months. CONCLUSIONS: The ability to image at depth, the phantom's robustness and flexible layered structure, and the ready incorporation of "optical markers" make these ideal depth standards for the validation of a variety of imaging modalities. | en_US |
dc.format.extent | 126007 - ? | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | J Biomed Opt | en_US |
dc.rights | Published by SPIE under a Creative Commons Attribution 4.0 International License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. | |
dc.subject | axial scaling | en_US |
dc.subject | depth imaging | en_US |
dc.subject | hydrogel | en_US |
dc.subject | non-linear imaging | en_US |
dc.subject | phantoms | en_US |
dc.subject | standards | en_US |
dc.subject | Hydrogels | en_US |
dc.subject | Phantoms, Imaging | en_US |
dc.subject | Microscopy | en_US |
dc.subject | Optical Imaging | en_US |
dc.subject | Optical Devices | en_US |
dc.title | Development of hydrogel-based standards and phantoms for non-linear imaging at depth. | en_US |
dc.type | Article | |
dc.rights.holder | © The Authors. | |
dc.identifier.doi | 10.1117/1.JBO.28.12.126007 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/38155703 | en_US |
pubs.issue | 12 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 28 | en_US |
dcterms.dateAccepted | 2023-08-28 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |
qmul.funder | Lighting the Way to a Healthy Nation - Optical 'X-rays' for Walk Through Diagnosis & Therapy::Engineering and Physical Sciences Research Council | en_US |