dc.contributor.author | Lewis, FC | |
dc.contributor.author | Bryan, N | |
dc.contributor.author | Hunt, JA | |
dc.date.accessioned | 2021-06-04T11:36:47Z | |
dc.date.available | 2012-06-29 | |
dc.date.available | 2021-06-04T11:36:47Z | |
dc.date.issued | 2012-08-08 | |
dc.identifier.issn | 2045-9769 | |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/72325 | |
dc.description.abstract | BACKGROUND: Human embryonic stem cells (hESCs) represent a tremendous resource for cell therapies and the study of human development; however to maintain their undifferentiated state in vitro they routinely require the use of mouse embryonic fibroblast (MEF) feeder-layers and exogenous protein media supplementation. RESULTS: These well established requirements can be overcome and in this study, it will be demonstrated that phenotypic stability of hESCs can be maintained using a novel, human plasma protein-based hydrogel as an extracellular culture matrix without the use of feeder cell co-culture. hESCs were resuspended in human platelet poor plasma (PPP), which was gelled by the addition of calcium containing DMEM-based hESC culture medium. Phenotypic and genomic expression of the pluripotency markers OCT4, NANOG and SOX2 were measured using immunohistochemistry and qRT-PCR respectively. Typical hESC morphology was demonstrated throughout in vitro culture and both viability and phenotypic stability were maintained throughout extended culture, up to 25 passages. CONCLUSIONS: PPP-derived hydrogel has demonstrated to be an efficacious alternative to MEF co-culture with its hydrophilicity allowing for this substrate to be delivered via minimally invasive procedures in a liquid phase with polymerization ensuing in situ. Together this provides a novel technique for the study of this unique group of stem cells in either 2D or 3D both in vitro and in vivo. | en_US |
dc.format.extent | 6 | |
dc.language | eng | |
dc.publisher | BioMed Central Ltd. | en_US |
dc.relation.ispartof | Cell Regen | |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |
dc.subject | Cell culture | en_US |
dc.subject | Embryonic stem cells | en_US |
dc.subject | Hydrogel | en_US |
dc.subject | Pluripotent stem cells | en_US |
dc.title | A feeder-free, human plasma-derived hydrogel for maintenance of a human embryonic stem cell phenotype in vitro. | en_US |
dc.type | Article | en_US |
dc.rights.holder | © 2012 Lewis et al.; licensee BioMed Central Ltd. | |
dc.identifier.doi | 10.1186/2045-9769-1-6 | |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/25408869 | en_US |
pubs.issue | 1 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published online | en_US |
pubs.volume | 1 | en_US |
dcterms.dateAccepted | 2012-06-29 | |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |