dc.contributor.author | Booth, LC | |
dc.contributor.author | Yao, ST | |
dc.contributor.author | Korsak, A | |
dc.contributor.author | Farmer, DGS | |
dc.contributor.author | Hood, SG | |
dc.contributor.author | McCormick, D | |
dc.contributor.author | Boesley, Q | |
dc.contributor.author | Connelly, AA | |
dc.contributor.author | McDougall, SJ | |
dc.contributor.author | Korim, WS | |
dc.contributor.author | Guild, S-J | |
dc.contributor.author | Mastitskaya, S | |
dc.contributor.author | Le, P | |
dc.contributor.author | Teschemacher, AG | |
dc.contributor.author | Kasparov, S | |
dc.contributor.author | Ackland, GL | |
dc.contributor.author | Malpas, SC | |
dc.contributor.author | McAllen, RM | |
dc.contributor.author | Allen, AM | |
dc.contributor.author | May, CN | |
dc.contributor.author | Gourine, AV | |
dc.date.accessioned | 2021-04-13T15:35:13Z | |
dc.date.available | 2020-11-11 | |
dc.date.available | 2021-04-13T15:35:13Z | |
dc.date.issued | 2020-11-17 | |
dc.identifier.citation | Lindsea C. Booth, Song T. Yao, Alla Korsak, David G.S. Farmer, Sally G. Hood, Daniel McCormick, Quinn Boesley, Angela A. Connelly, Stuart J. McDougall, Willian S. Korim, Sarah-Jane Guild, Svetlana Mastitskaya, Phuong Le, Anja G. Teschemacher, Sergey Kasparov, Gareth L. Ackland, Simon C. Malpas, Robin M. McAllen, Andrew M. Allen, Clive N. May, Alexander V. Gourine, Selective optogenetic stimulation of efferent fibers in the vagus nerve of a large mammal, Brain Stimulation, Volume 14, Issue 1, 2021, Pages 88-96, doi: 10.1016/j.brs.2020.11.010 | en_US |
dc.identifier.issn | 1935-861X | |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/71245 | |
dc.description.abstract | Background: Electrical stimulation applied to individual organs, peripheral nerves, or specific brain regions has been used to treat a range of medical conditions. In cardiovascular disease, autonomic
dysfunction contributes to the disease progression and electrical stimulation of the vagus nerve has been
pursued as a treatment for the purpose of restoring the autonomic balance. However, this approach lacks
selectivity in activating function- and organ-specific vagal fibers and, despite promising results of many
preclinical studies, has so far failed to translate into a clinical treatment of cardiovascular disease.
Objective: Here we report a successful application of optogenetics for selective stimulation of vagal
efferent activity in a large animal model (sheep).
Methods and results: Twelve weeks after viral transduction of a subset of vagal motoneurons, strong
axonal membrane expression of the excitatory light-sensitive ion channel ChIEF was achieved in the
efferent projections innervating thoracic organs and reaching beyond the level of the diaphragm. Blue
laser or LED light (>10 mW mm 2
; 1 ms pulses) applied to the cervical vagus triggered precisely timed,
strong bursts of efferent activity with evoked action potentials propagating at speeds of ~6 m s 1
.
Conclusions: These findings demonstrate that in species with a large, multi-fascicled vagus nerve, it is
possible to stimulate a specific sub-population of efferent fibers using light at a site remote from the
vector delivery, marking an important step towards eventual clinical use of optogenetic technology for
autonomic neuromodulation.
© 2020 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license
(http://creativecommons.org/licenses/by/4.0/) | en_US |
dc.format.extent | 88 - 96 | |
dc.language.iso | en | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.ispartof | BRAIN STIMULATION | |
dc.rights | CC BY license | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Autonomic nervous system | en_US |
dc.subject | Brainstem | en_US |
dc.subject | Neuromodulation | en_US |
dc.subject | Optogenetic | en_US |
dc.subject | Vagal preganglionic neurons | en_US |
dc.subject | Vagus nerve stimulation | en_US |
dc.title | Selective optogenetic stimulation of efferent fi bers in the vagus nerve of a large mammal | en_US |
dc.type | Article | en_US |
dc.rights.holder | © 2020 The Author(s). Published by Elsevier Inc. | |
dc.identifier.doi | 10.1016/j.brs.2020.11.010 | |
pubs.author-url | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000614508100015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=612ae0d773dcbdba3046f6df545e9f6a | en_US |
pubs.issue | 1 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 14 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |
qmul.funder | Understanding vagal control of the left ventricle::British Heart Foundation Programme Grant | en_US |