Show simple item record

dc.contributor.authorOmbrato, L
dc.contributor.authorNolan, E
dc.contributor.authorPassaro, D
dc.contributor.authorKurelac, I
dc.contributor.authorBridgeman, VL
dc.contributor.authorWaclawiczek, A
dc.contributor.authorDuarte, D
dc.contributor.authorLo Celso, C
dc.contributor.authorBonnet, D
dc.contributor.authorMalanchi, I
dc.date.accessioned2020-12-18T18:06:47Z
dc.date.available2020-10-08
dc.date.available2020-12-18T18:06:47Z
dc.date.issued2020-12-11
dc.identifier.citationOmbrato, L., Nolan, E., Passaro, D. et al. Generation of neighbor-labeling cells to study intercellular interactions in vivo. Nat Protoc (2020). https://doi.org/10.1038/s41596-020-00438-5en_US
dc.identifier.urihttps://qmro.qmul.ac.uk/xmlui/handle/123456789/69458
dc.description.abstractUnderstanding cell-cell interactions is critical in most, if not all, research fields in biology. Nevertheless, studying intercellular crosstalk in vivo remains a relevant challenge, due mainly to the difficulty in spatially locating the surroundings of particular cells in the tissue. Cherry-niche is a powerful new method that enables cells expressing a fluorescent protein to label their surrounding cells, facilitating their specific isolation from the whole tissue as live cells. We previously applied Cherry-niche in cancer research to study the tumor microenvironment (TME) in metastasis. Here we describe how to generate cancer cells with the ability to label their neighboring cells (within the tumor niche) by transferring a liposoluble fluorescent protein. Live niche cells can be isolated and compared with cells distant from the tumor bulk, using a variety of ex vivo approaches. As previously shown, this system has the potential to identify novel components in the TME and improve our understanding of their local interactions. Importantly, Cherry-niche can also be applied to study potential cell-cell interactions due to in vivo proximity in research fields beyond cancer. This protocol takes 2-3 weeks to generate the labeling cells and 1-2 weeks to test their labeling ability.en_US
dc.languageeng
dc.publisherSpringer Natureen_US
dc.relation.ispartofNature Protocols
dc.rightsPost-prints subject Springer Nature re-use terms
dc.titleGeneration of neighbor-labeling cells to study intercellular interactions in vivo.en_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41596-020-00438-5
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/33311715en_US
pubs.notesNot knownen_US
pubs.publication-statusPublished onlineen_US
dcterms.dateAccepted2020-10-08
rioxxterms.funderDefault funderen_US
rioxxterms.identifier.projectDefault projecten_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record