A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue
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Volume
44
Pagination
109 - 111
Publisher
DOI
10.1016/j.placenta.2016.04.014
Journal
PLACENTA
ISSN
0143-4004
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Show full item recordAbstract
A rapid method to determine quality for placental proteomic studies is required due to varying lengths of time between delivery and sampling in routine protocols. We developed a rapid 10 min LC-MS based scanning method to profile free peptides liberated from natural proteolytic degradation. The assay was applied to placenta samples obtained following refrigeration for varying time periods post-delivery (12 h, +24 h, +48 h and +72 h). Analysis reveals time dependant overlapping profiles for groups <24 to +48 h with greatest variation in the +72 h group, indicating that significant proteolysis affects tissue integrity between 48 and 72 h.
Authors
Heywood, WE; Pryce, J; Virasami, A; Preece, RL; Dezateux, C; Mills, K; Sebire, NJCollections
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