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A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue

dc.contributor.authorHeywood, WE
dc.contributor.authorPryce, J
dc.contributor.authorVirasami, A
dc.contributor.authorPreece, RL
dc.contributor.authorDezateux, C
dc.contributor.authorMills, K
dc.contributor.authorSebire, NJ
dc.date.accessioned2019-02-26T15:46:12Z
dc.date.available2016-04-15
dc.date.available2019-02-26T15:46:12Z
dc.date.issued2016-04-30
dc.identifier.citationHeywood, W. E., et al. (2016). "A rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissue." Placenta 44: 109-111.en_US
dc.identifier.issn0143-4004
dc.identifier.urihttps://qmro.qmul.ac.uk/xmlui/handle/123456789/55559
dc.description.abstractA rapid method to determine quality for placental proteomic studies is required due to varying lengths of time between delivery and sampling in routine protocols. We developed a rapid 10 min LC-MS based scanning method to profile free peptides liberated from natural proteolytic degradation. The assay was applied to placenta samples obtained following refrigeration for varying time periods post-delivery (12 h, +24 h, +48 h and +72 h). Analysis reveals time dependant overlapping profiles for groups <24 to +48 h with greatest variation in the +72 h group, indicating that significant proteolysis affects tissue integrity between 48 and 72 h.en_US
dc.description.sponsorshipThis work was supported by the Economic and Social Research Council (ESRC) [Grant numbers ES/J007501/1, ES/L002507/1, ES/L002353/1, ES/L012871/1, ES/N007549/1] and as part of the GOSomics initiative at the National Institute for Health Research (NIHR) Biomedical Research Centre at Great Ormond Street Hospital for Children NHS Foundation Trust and University College London and the UCL Biological Mass Spectrometry Research Centreen_US
dc.format.extent109 - 111
dc.language.isoenen_US
dc.publisherElsevier/Science Directen_US
dc.relation.ispartofPLACENTA
dc.rightsCreative Commons Attribution
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.subjectPlacentaen_US
dc.subjectProteolysisen_US
dc.subjectMultivariate analysisen_US
dc.subjectLC-MSen_US
dc.subjectFree peptideen_US
dc.titleA rapid high throughput proteomic method based on profiling of proteolytic free peptides to assess post-delivery degradation of placental tissueen_US
dc.typeArticleen_US
dc.rights.holder2016 The Authors
dc.identifier.doi10.1016/j.placenta.2016.04.014
pubs.author-urlhttp://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000381650900016&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=612ae0d773dcbdba3046f6df545e9f6aen_US
pubs.notesNot knownen_US
pubs.publication-statusPublisheden_US
pubs.volume44en_US
dcterms.dateAccepted2016-04-15
rioxxterms.funderDefault funderen_US
rioxxterms.identifier.projectDefault projecten_US


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