dc.contributor.author | LEE, WJ | |
dc.contributor.author | Fu, RM | |
dc.contributor.author | Liang, C | |
dc.contributor.author | SLOAN, RD | |
dc.date.accessioned | 2019-02-08T16:27:05Z | |
dc.date.available | 2018-09-11 | |
dc.date.available | 2019-02-08T16:27:05Z | |
dc.date.issued | 2018-09-28 | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/55256 | |
dc.description.abstract | Interferon induced transmembrane proteins (IFITMs) inhibit the cellular entry of a broad range of viruses, but it has been suspected that for HIV-1 IFITMs may also inhibit a post-integration replicative step. We show that IFITM expression reduces HIV-1 viral protein synthesis by preferentially excluding viral mRNA transcripts from translation and thereby restricts viral production. Codon-optimization of proviral DNA rescues viral translation, implying that IFITM-mediated restriction requires recognition of viral RNA elements. In addition, we find that expression of the viral accessory protein Nef can help overcome the IFITM-mediated inhibition of virus production. Our studies identify a novel role for IFITMs in inhibiting HIV replication at the level of translation, but show that the effects can be overcome by the lentiviral protein Nef. | en_US |
dc.description.sponsorship | Wellcome Trust-University of Edinburgh Institutional Strategic Support Fund | en_US |
dc.language.iso | en | en_US |
dc.publisher | Nature Publishing Group | en_US |
dc.relation.ispartof | Scientific Reports | |
dc.rights | Creative Commons Attribution 4.0 International License | |
dc.title | IFITM proteins inhibit HIV-1 protein synthesis | en_US |
dc.type | Article | en_US |
dc.rights.holder | © The Author(s) 2018. | |
dc.identifier.doi | 10.1038/s41598-018-32785-5 | |
pubs.notes | No embargo | en_US |
pubs.publication-status | Published | en_US |
dcterms.dateAccepted | 2018-09-11 | |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |