dc.contributor.author | Pan, Y | en_US |
dc.contributor.author | Thapa, D | en_US |
dc.contributor.author | Baldissera, L | en_US |
dc.contributor.author | Argunhan, F | en_US |
dc.contributor.author | Aubdool, AA | en_US |
dc.contributor.author | Brain, SD | en_US |
dc.date.accessioned | 2018-08-07T10:58:29Z | |
dc.date.available | 2017-10-30 | en_US |
dc.date.issued | 2018-05 | en_US |
dc.date.submitted | 2018-07-19T10:09:11.314Z | |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/43088 | |
dc.description.abstract | Cold exposure is directly related to skin conditions, such as frostbite. This is due to the cold exposure inducing a vasoconstriction to reduce cutaneous blood flow and protect against heat loss. However, a long-term constriction will cause ischaemia and potentially irreversible damage. We have developed techniques to elucidate the mechanisms of the vascular cold response. We focused on two ligand-gated transient receptor potential (TRP) channels, namely, the established "cold sensors" TRP ankyrin 1 (TRPA1) and TRP melastin (TRPM8). We used the anaesthetised mouse and measured cutaneous blood flow by laser speckle imaging. Two cold treatments were used. A generalised cold treatment was achieved through whole paw water immersion (10 °C for 5 min) and a localised cold treatment that will be potentially easier to translate to human studies was carried out on the mouse paw with a copper cold probe (0.85-cm diameter). The results show that TRPA1 and TRPM8 can each act as a vascular cold sensor to mediate the vasoconstrictor component of whole paw cooling as expected from our previous research. However, the local cooling-induced responses were only blocked when the TRPA1 and TRPM8 antagonists were given simultaneously. This suggests that this localised cold probe response requires both functional TRPA1 and TRPM8. | en_US |
dc.description.sponsorship | We thank the Biotechnology and Biological Sciences Research Council (BBSRC), (DT) British Heart Foundation (BHF) (AA), Medical Research Council (MRC) (FA) and Science without Borders (Brazilian government; LB) for funding. Y Pan was a KCL MSc Pharmacology student. | en_US |
dc.format.extent | 779 - 786 | en_US |
dc.language | eng | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Pflugers Arch | en_US |
dc.subject | Blood flow | en_US |
dc.subject | Cold | en_US |
dc.subject | TRPA1 | en_US |
dc.subject | TRPM8 | en_US |
dc.subject | Thermoreceptors | en_US |
dc.subject | Vascular | en_US |
dc.subject | Animals | en_US |
dc.subject | Cold Temperature | en_US |
dc.subject | Male | en_US |
dc.subject | Mice | en_US |
dc.subject | Microvessels | en_US |
dc.subject | Skin | en_US |
dc.subject | TRPA1 Cation Channel | en_US |
dc.subject | TRPM Cation Channels | en_US |
dc.subject | Thermosensing | en_US |
dc.subject | Vasoconstriction | en_US |
dc.title | Relevance of TRPA1 and TRPM8 channels as vascular sensors of cold in the cutaneous microvasculature. | en_US |
dc.type | Article | |
dc.identifier.doi | 10.1007/s00424-017-2085-9 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/29164310 | en_US |
pubs.issue | 5 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 470 | en_US |
dcterms.dateAccepted | 2017-10-30 | en_US |