dc.contributor.author | Abdul Momin, MHF | en_US |
dc.contributor.author | Bean, DC | en_US |
dc.contributor.author | Hendriksen, RS | en_US |
dc.contributor.author | Haenni, M | en_US |
dc.contributor.author | Phee, LM | en_US |
dc.contributor.author | Wareham, DW | en_US |
dc.date.accessioned | 2018-04-20T16:46:43Z | |
dc.date.available | 2017-09-06 | en_US |
dc.date.issued | 2017-11 | en_US |
dc.date.submitted | 2018-02-26T11:24:50.204Z | |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/36434 | |
dc.description.abstract | PURPOSE: A selective chromogenic culture medium for the laboratory isolation and differentiation of colistin resistant Acinetobacter, Pseudomonas, Stenotrophomonas and Enterobacteriaceae spp. (CHROMagar COL-APSE) was developed, evaluated and compared to an existing selective bacterial culture medium (SuperPolymyxin). METHODOLOGY: The medium was challenged with 84 isolates, including polymyxin B (POL B)-susceptible and -resistant type strains and colistin (COL)-resistant organisms recovered from human and animal samples. Susceptibility to COL and POL B was determined by agar dilution and broth microtitre dilution. The lower limit for the detection of COL-resistant organisms was also calculated for both CHROMagar COL-APSE and SuperPolymyxin media. The ability to isolate and correctly differentiate COL-resistant organisms within mixed cultures was also assessed and compared using both media. RESULTS: Using CHROMagar COL-APSE, Gram-negative pathogens (n=71) with intrinsic (n=8) or acquired COL (n=63) resistance were recovered with 100 % specificity down to the lower limit of detection of 101 colony-forming units (c.f.u.). The growth on SuperPolymyxin was similar, but notably weaker for COL-resistant non-fermentative bacteria (Acinetobacter, Pseudomonas and Stenotrophomonas). CHROMagar COL-APSE was also more sensitive in supporting the growth of Enterobacteriaceae with COL resistance associated with the carriage of mcr-1. CONCLUSION: CHROMagar COL-APSE is a sensitive and specific medium for the growth of COL-resistant bacterial pathogens. Due to the low limit of detection (101 c.f.u.), it may be useful as a primary isolation medium in the surveillance and recovery of COL-resistant bacteria from complex human, veterinary and environmental samples, especially those with plasmid-mediated MCR-1 or novel mechanisms of polymyxin resistance. | en_US |
dc.format.extent | 1554 - 1561 | en_US |
dc.language | eng | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | J Med Microbiol | en_US |
dc.rights | Non-commercial use. | |
dc.rights | The final publication is published online https://dx.doi.org/10.1099/jmm.0.000602 | |
dc.subject | CHROMagar COL-APSE | en_US |
dc.subject | MCR-1 | en_US |
dc.subject | colistin | en_US |
dc.subject | resistance | en_US |
dc.subject | Anti-Bacterial Agents | en_US |
dc.subject | Chromogenic Compounds | en_US |
dc.subject | Colistin | en_US |
dc.subject | Culture Media | en_US |
dc.subject | Drug Resistance, Bacterial | en_US |
dc.subject | Gram-Negative Bacteria | en_US |
dc.subject | Microbial Sensitivity Tests | en_US |
dc.title | CHROMagar COL-APSE: a selective bacterial culture medium for the isolation and differentiation of colistin-resistant Gram-negative pathogens. | en_US |
dc.type | Article | |
dc.rights.holder | © 2017 The Authors. | |
dc.identifier.doi | 10.1099/jmm.0.000602 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/28984232 | en_US |
pubs.issue | 11 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 66 | en_US |