dc.contributor.author | Anandasabapathy, N | en_US |
dc.contributor.author | Feder, R | en_US |
dc.contributor.author | Mollah, S | en_US |
dc.contributor.author | Tse, S-W | en_US |
dc.contributor.author | Longhi, MP | en_US |
dc.contributor.author | Mehandru, S | en_US |
dc.contributor.author | Matos, I | en_US |
dc.contributor.author | Cheong, C | en_US |
dc.contributor.author | Ruane, D | en_US |
dc.contributor.author | Brane, L | en_US |
dc.contributor.author | Teixeira, A | en_US |
dc.contributor.author | Dobrin, J | en_US |
dc.contributor.author | Mizenina, O | en_US |
dc.contributor.author | Park, CG | en_US |
dc.contributor.author | Meredith, M | en_US |
dc.contributor.author | Clausen, BE | en_US |
dc.contributor.author | Nussenzweig, MC | en_US |
dc.contributor.author | Steinman, RM | en_US |
dc.date.accessioned | 2018-02-21T14:31:08Z | |
dc.date.issued | 2014-08-25 | en_US |
dc.date.submitted | 2018-02-21T09:46:26.981Z | |
dc.identifier.issn | 0022-1007 | en_US |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/33083 | |
dc.description.abstract | <jats:p>DCs are critical for initiating immunity. The current paradigm in vaccine biology is that DCs migrating from peripheral tissue and classical lymphoid-resident DCs (cDCs) cooperate in the draining LNs to initiate priming and proliferation of T cells. Here, we observe subcutaneous immunity is Fms-like tyrosine kinase 3 ligand (Flt3L) dependent. Flt3L is rapidly secreted after immunization; Flt3 deletion reduces T cell responses by 50%. Flt3L enhances global T cell and humoral immunity as well as both the numbers and antigen capture capacity of migratory DCs (migDCs) and LN-resident cDCs. Surprisingly, however, we find immunity is controlled by cDCs and actively tempered in vivo by migDCs. Deletion of Langerin+ DC or blockade of DC migration improves immunity. Consistent with an immune-regulatory role, transcriptomic analyses reveals different skin migDC subsets in both mouse and human cluster together, and share immune-suppressing gene expression and regulatory pathways. These data reveal that protective immunity to protein vaccines is controlled by Flt3L-dependent, LN-resident cDCs.</jats:p> | en_US |
dc.format.extent | 1875 - 1891 | en_US |
dc.language | en | en_US |
dc.language.iso | en | en_US |
dc.publisher | Rockefeller University Press | en_US |
dc.relation.ispartof | Journal of Experimental Medicine | en_US |
dc.rights | CC-BY--NC-SA | |
dc.title | Classical Flt3L-dependent dendritic cells control immunity to protein vaccine | en_US |
dc.type | Article | |
dc.rights.holder | © 2014 Anandasabapathy et al. | |
dc.identifier.doi | 10.1084/jem.20131397 | en_US |
pubs.issue | 9 | en_US |
pubs.notes | No embargo | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 211 | en_US |