Browsing Centre for Microvascular Research (CMR) by Author "Barkaway, A"
Now showing items 1-8 of 8
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Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage
Barkaway, A; Rolas, L; Joulia, R; Bodkin, J; Lenn, T; Owen-Woods, C; Reglero-Real, N; Stein, M; Vazquez Martinez, L; Girbl, T (Elsevier Inc., 2021-05-24)Aging is associated with dysregulated immune functions. Here, we investigated the impact of age on neutrophil diapedesis. Using confocal intravital microscopy, we found that in inflamed aged tissues neutrophils exhibited ... -
Autophagy modulates endothelial junctions to restrain neutrophil diapedesis during inflammation
Reglero, N; Pérez-Gutiérrez, L; Yoshimura, A; Rolas, L; Garrido-Mesa, J; Barkaway, A; PICKWORTH, C; Saleeb, R; Gonzalez-Nuñez, M; Austin-Williams, SN (Elsevier, 2021-08-06)The migration of neutrophils from the blood circulation to sites of infection or injury is a key immune response and requires the breaching of endothelial cells (ECs) that line the inner aspect of blood vessels. ... -
Cardiovascular Progerin Suppression and lamin A Restoration Rescues Hutchinson-Gilford Progeria Syndrome.
Sánchez-López, A; Espinós-Estévez, C; González-Gómez, C; Gonzalo, P; Andrés-Manzano, MJ; Fanjul, V; Riquelme-Borja, R; Hamczyk, MR; Macías, Á; Del Campo, L (Wolters Kluwer Health, Inc., 2021-10-25)Background: Hutchinson-Gilford progeria syndrome (HGPS) is a rare disorder characterized by premature aging and death mainly due to myocardial infarction, stroke, or heart failure. The disease is provoked by progerin, a ... -
Development of a CRISPR/Cas9-based therapy for Hutchinson-Gilford progeria syndrome
NOURSHARGH, S; Santiago-Fernández, O; Osorio, F G; Quesada, V; Rodríguez, F; Basso, S; Maeso, D; Rolas, L; Barkaway, A; Folgueras, A R (Nature Research, 2019-02-18)CRISPR/Cas9-based therapies hold considerable promise for the treatment of genetic diseases. Among these, Hutchinson–Gilford progeria syndrome, caused by a point mutation in the LMNA gene, stands out as a potential candidate. ... -
Distinct Compartmentalization of the Chemokines CXCL1 and CXCL2 and the Atypical Receptor ACKR1 Determine Discrete Stages of Neutrophil Diapedesis.
Girbl, T; Lenn, T; Perez, L; Rolas, L; Barkaway, A; Thiriot, A; Del Fresno, C; Lynam, E; Hub, E; Thelen, M (2018-12-18)Neutrophils require directional cues to navigate through the complex structure of venular walls and into inflamed tissues. Here we applied confocal intravital microscopy to analyze neutrophil emigration in cytokine-stimulated ... -
Isoprenylcysteine Carboxylmethyltransferase-Based Therapy for Hutchinson-Gilford Progeria Syndrome
Marcos-Ramiro, B; Gil-Ordonez, A; Marin-Ramos, NI; Ortega-Nogales, FJ; Balabasquer, M; Gonzalo, P; Khiar-Fernandez, N; Rolas, L; Barkaway, A; Nourshargh, S (2021) -
Local microvascular leakage promotes trafficking of activated neutrophils to remote organs.
Owen-Woods, C; Joulia, R; Barkaway, A; Rolas, L; Ma, B; Nottebaum, AF; Arkill, KP; Stein, M; Girbl, T; Golding, M (2020-03-23)Increased microvascular permeability to plasma proteins and neutrophil emigration are hallmarks of innate immunity and key features of numerous inflammatory disorders. Although neutrophils can promote microvascular leakage, ... -
Senescent endothelial cells promote pathogenic neutrophil trafficking in inflamed tissues
Rolas, L; Stein, M; Barkaway, A; Reglero, N; Sciacca, E; Yaseen, M; Wang, H; Vazquez-Martinez, L; Golding, M; Blacksell, IA (EMBO Press, 2024-06-25)Cellular senescence is a hallmark of advanced age and a major instigator of numerous inflammatory pathologies. Whilst endothelial cell (EC) senescence is aligned with defective vascular functionality, its impact on fundamental ...