Browsing Chemistry and Biochemistry by Author "Thorpe, P"
Now showing items 1-2 of 2
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Automated Fluorescence Lifetime Imaging High-Content Analysis of Förster Resonance Energy Transfer between Endogenously Labeled Kinetochore Proteins in Live Budding Yeast Cells.
Guo, W; Kumar, S; Görlitz, F; Garcia, E; Alexandrov, Y; Munro, I; Kelly, DJ; Warren, S; Thorpe, P; Dunsby, C (SAGE, 2019-01-10)We describe an open-source automated multiwell plate fluorescence lifetime imaging (FLIM) methodology to read out Förster resonance energy transfer (FRET) between fluorescent proteins (FPs) labeling endogenous kinetochore ... -
Bringing Functional Genomics into Focus
Ledesma-Fernández, E; Thorpe, P; de Bruin, R (Elsevier, 2016-09)Styles et al. develop an optimized method that combines high-content microscopy and automated phenotypic analysis with genome-wide yeast genetics to identify genes in DNA damage repair.