dc.contributor.author | Gould, D | en_US |
dc.date.accessioned | 2017-09-19T09:16:35Z | |
dc.date.issued | 2017 | en_US |
dc.date.submitted | 2017-09-01T14:21:08.406Z | |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/25768 | |
dc.description.abstract | Before designing a synthetic promoter, it can be helpful to think about its final application. Is the study purely an in vitro exercise in monitoring short-term promoter activity from an episomal vector, or does the promoter eventually need to be permanently active and be integrated into the genome or perhaps even to function in vivo? The final application will have a bearing on promoter design and vector of choice from the start of the study. In this chapter I highlight some of the vector attributes to consider and features that should be thought about. | en_US |
dc.format.extent | 1 - 7 | en_US |
dc.rights | The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-4939-7223-4 | |
dc.subject | Amplification | en_US |
dc.subject | Immunogenicity | en_US |
dc.subject | Regulated promoters | en_US |
dc.subject | Replication | en_US |
dc.subject | Reporter genes | en_US |
dc.subject | Animals | en_US |
dc.subject | Cloning, Molecular | en_US |
dc.subject | Gene Expression Regulation | en_US |
dc.subject | Genes, Reporter | en_US |
dc.subject | Genetic Therapy | en_US |
dc.subject | Genetic Vectors | en_US |
dc.subject | Humans | en_US |
dc.subject | Immunogenetic Phenomena | en_US |
dc.subject | Plasmids | en_US |
dc.subject | Promoter Regions, Genetic | en_US |
dc.subject | Synthetic Biology | en_US |
dc.title | Initial Considerations Before Designing a Promoter Construct. | en_US |
dc.type | Book chapter | |
dc.rights.holder | © Springer Science+Business Media LLC 2017 | |
dc.identifier.doi | 10.1007/978-1-4939-7223-4_1 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/28801895 | en_US |
pubs.notes | Not known | en_US |
pubs.notes | This is the submitted chapter | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 1651 | en_US |