Isolation of bacterial extrachromosomal DNA from human dental plaque associated with periodontal disease, using transposon-aided capture (TRACA).
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Published version
Embargoed until: 2100-01-01
Reason: Publisher version
Embargoed until: 2100-01-01
Reason: Publisher version
Volume
78
Pagination
349 - 354
DOI
10.1111/j.1574-6941.2011.01166.x
Journal
FEMS Microbiol Ecol
Issue
Metadata
Show full item recordAbstract
The human oral cavity is host to a complex microbial community estimated to comprise >700 bacterial species, of which at least half are thought to be not yet cultivable in vitro. To investigate the plasmids present in this community, we used a transposon-aided capture system, which allowed the isolation of plasmids from human oral supra- and subgingival plaque samples. Thirty-two novel plasmids and a circular molecule that could be an integrase-generated circular intermediate were isolated.
Authors
Warburton, PJ; Allan, E; Hunter, S; Ward, J; Booth, V; Wade, WG; Mullany, PCollections
- Centre for Immunobiology [1029]