dc.contributor.author | THORPE, SD | en_US |
dc.contributor.author | Buckley, CT | en_US |
dc.contributor.author | Kelly, DJ | en_US |
dc.contributor.editor | El Haj, E | en_US |
dc.contributor.editor | Bader, D | en_US |
dc.date.accessioned | 2016-01-15T12:02:54Z | |
dc.date.issued | 2011 | en_US |
dc.date.submitted | 2015-12-04T17:53:53.543Z | |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/10719 | |
dc.description.abstract | The objectives of this study were twofold; to determine if cartilage specific matrix synthesis by mesenchymal stem cells (MSCs) is regulated by the magnitude and/or duration of dynamic compression in the absence of growth factors, and to investigate if expanding MSCs in the presence of both fibroblast growth factor-2 (FGF-2) and transforming growth factor β-3 (TGF-β3) would influence their subsequent response to dynamic compression following encapsulation in agarose hydrogels. Porcine bone marrow derived MSCs were suspended in agarose and cast to produce cylinders (Ø5×3mm). Constructs were maintained in a chemically defined medium. Dynamic compression was applied at 1 Hz at strain amplitudes of 5%, 10% and 5% superimposed upon a 5% pre-strain for durations of 1, 3 and 12 hours. MSCs were also expanded in the presence of FGF-2 and TGF-β3. The biochemical constituents of constructs were analyzed. Under strain magnitudes of 5% and 10% and durations of 1 and 3 hours small increases in sGAG accumulation relative to unloaded controls were observed. However this was orders of magnitude lower than that induced by TGF-β3 stimulation. Expansion in FGF-2 and TGF-β3 did not positively modulate chondrogenesis of MSCs in either unloaded or loaded culture. | en_US |
dc.format.extent | 43 - 46 (4) | en_US |
dc.publisher | Springer Berlin Heidelberg | en_US |
dc.rights | • “The final publication is available at Springer via http://dx.doi.org/10.1007/978-3-642-19044-5_12” | |
dc.title | Can Dynamic Compression in the Absence of Growth Factors Induce Chondrogenic Differentiation of Bone Marrow Derived MSCs Encapsulated in Agarose Hydrogels? | en_US |
dc.type | Article | |
dc.rights.holder | © Springer-Verlag Berlin Heidelberg 2011 | |
dc.identifier.doi | 10.1007/978-3-642-19044-5_12 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 30 | en_US |