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    Can Dynamic Compression in the Absence of Growth Factors Induce Chondrogenic Differentiation of Bone Marrow Derived MSCs Encapsulated in Agarose Hydrogels? 
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    • Can Dynamic Compression in the Absence of Growth Factors Induce Chondrogenic Differentiation of Bone Marrow Derived MSCs Encapsulated in Agarose Hydrogels?
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    • Can Dynamic Compression in the Absence of Growth Factors Induce Chondrogenic Differentiation of Bone Marrow Derived MSCs Encapsulated in Agarose Hydrogels?
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    Can Dynamic Compression in the Absence of Growth Factors Induce Chondrogenic Differentiation of Bone Marrow Derived MSCs Encapsulated in Agarose Hydrogels?

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    Accepted version (200.7Kb)
    Editors
    El Haj, E
    Bader, D
    Volume
    30
    Pagination
    43 - 46 (4)
    Publisher
    Springer Berlin Heidelberg
    DOI
    10.1007/978-3-642-19044-5_12
    Metadata
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    Abstract
    The objectives of this study were twofold; to determine if cartilage specific matrix synthesis by mesenchymal stem cells (MSCs) is regulated by the magnitude and/or duration of dynamic compression in the absence of growth factors, and to investigate if expanding MSCs in the presence of both fibroblast growth factor-2 (FGF-2) and transforming growth factor β-3 (TGF-β3) would influence their subsequent response to dynamic compression following encapsulation in agarose hydrogels. Porcine bone marrow derived MSCs were suspended in agarose and cast to produce cylinders (Ø5×3mm). Constructs were maintained in a chemically defined medium. Dynamic compression was applied at 1 Hz at strain amplitudes of 5%, 10% and 5% superimposed upon a 5% pre-strain for durations of 1, 3 and 12 hours. MSCs were also expanded in the presence of FGF-2 and TGF-β3. The biochemical constituents of constructs were analyzed. Under strain magnitudes of 5% and 10% and durations of 1 and 3 hours small increases in sGAG accumulation relative to unloaded controls were observed. However this was orders of magnitude lower than that induced by TGF-β3 stimulation. Expansion in FGF-2 and TGF-β3 did not positively modulate chondrogenesis of MSCs in either unloaded or loaded culture.
    Authors
    THORPE, SD; Buckley, CT; Kelly, DJ
    URI
    http://qmro.qmul.ac.uk/xmlui/handle/123456789/10719
    Collections
    • Cell and Molecular Biology [192]
    Licence information
    • “The final publication is available at Springer via http://dx.doi.org/10.1007/978-3-642-19044-5_12”
    Copyright statements
    © Springer-Verlag Berlin Heidelberg 2011
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