dc.contributor.author | Cardosa, SR | en_US |
dc.contributor.author | Ogunkolade, BW | en_US |
dc.contributor.author | Lowe, R | en_US |
dc.contributor.author | Savage, E | en_US |
dc.contributor.author | Mein, CA | en_US |
dc.contributor.author | Boucher, BJ | en_US |
dc.contributor.author | Hitman, GA | en_US |
dc.date.accessioned | 2024-07-23T13:02:25Z | |
dc.date.available | 2021-07-26 | en_US |
dc.date.issued | 2021-08-14 | en_US |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/98355 | |
dc.description.abstract | BACKGROUND: Betel-nut consumption is the fourth most common addictive habit globally and there is good evidence linking the habit to obesity, type 2 diabetes (T2D) and the metabolic syndrome. The aim of our pilot study was to identify gene expression relevant to obesity, T2D and the metabolic syndrome using a genome-wide transcriptomic approach in a human monocyte cell line incubated with arecoline and its nitrosated products. RESULTS: The THP1 monocyte cell line was incubated separately with arecoline and 3-methylnitrosaminopropionaldehyde (MNPA) in triplicate for 24 h and pooled cDNA indexed paired-end libraries were sequenced (Illumina NextSeq 500). After incubation with arecoline and MNPA, 15 and 39 genes respectively had significant changes in their expression (q < 0.05, log fold change 1.5). Eighteen of those genes have reported associations with T2D and obesity in humans; of these genes there was most marked evidence for CLEC10A, MAPK8IP1, NEGR1, NQ01 and INHBE genes. CONCLUSIONS: Our preliminary studies have identified a large number of genes relevant to obesity, T2D and metabolic syndrome whose expression was changed significantly in human TPH1 cells following incubation with betel-nut derived arecoline or with MNPA. These findings require validation by further cell-based work and investigation amongst betel-chewing communities. | en_US |
dc.format.extent | 165 - ? | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | BMC Endocr Disord | en_US |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. | |
dc.subject | Betel-nut | en_US |
dc.subject | Obesity | en_US |
dc.subject | RNA-sequencing | en_US |
dc.subject | Transcriptomics | en_US |
dc.subject | Type 2 diabetes | en_US |
dc.subject | Areca | en_US |
dc.subject | Arecoline | en_US |
dc.subject | Biomarkers | en_US |
dc.subject | Diabetes Mellitus, Type 2 | en_US |
dc.subject | Follow-Up Studies | en_US |
dc.subject | Gene Expression Regulation | en_US |
dc.subject | Humans | en_US |
dc.subject | Metabolic Syndrome | en_US |
dc.subject | Monocytes | en_US |
dc.subject | Obesity | en_US |
dc.subject | Pilot Projects | en_US |
dc.subject | Prognosis | en_US |
dc.subject | Transcriptome | en_US |
dc.title | Areca catechu-(Betel-nut)-induced whole transcriptome changes in a human monocyte cell line that may have relevance to diabetes and obesity; a pilot study. | en_US |
dc.type | Article | |
dc.rights.holder | © The Author(s). 2021 | |
dc.identifier.doi | 10.1186/s12902-021-00827-1 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/34391409 | en_US |
pubs.issue | 1 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published online | en_US |
pubs.volume | 21 | en_US |
dcterms.dateAccepted | 2021-07-26 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |
rioxxterms.funder.project | b215eee3-195d-4c4f-a85d-169a4331c138 | en_US |