dc.contributor.author | Vester, SK | |
dc.contributor.author | Davies, AM | |
dc.contributor.author | Beavil, RL | |
dc.contributor.author | Sandhar, BS | |
dc.contributor.author | Beavil, AJ | |
dc.contributor.author | Gould, HJ | |
dc.contributor.author | Sutton, BJ | |
dc.contributor.author | McDonnell, JM | |
dc.date.accessioned | 2024-05-13T14:04:49Z | |
dc.date.available | 2023-11-10 | |
dc.date.available | 2024-05-13T14:04:49Z | |
dc.date.issued | 2023-11-16 | |
dc.identifier.citation | Vester, S.K.; Davies, A.M.; Beavil, R.L.; Sandhar, B.S.; Beavil, A.J.; Gould, H.J.; Sutton, B.J.; McDonnell, J.M. Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor. Antibodies 2023, 12, 75. https://doi.org/10.3390/antib12040075 | en_US |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/96843 | |
dc.description.abstract | We have previously produced a toolkit of antibodies, comprising recombinant human antibodies of all but one of the human isotypes, directed against the polcalcin family antigen Phl p 7. In this work, we complete the toolkit of human antibody isotypes with the IgD version of the anti-Phl p 7 monoclonal antibody. We also raised a set of nanobodies against the IgD anti-Phl p 7 antibody and identify and characterize one paratope-specific nanobody. This nanobody also binds to the IgE isotype of this antibody, which shares the same idiotype, and orthosterically inhibits the interaction with Phl p 7. The 2.1 Å resolution X-ray crystal structure of the nanobody in complex with the IgD Fab is described. | en_US |
dc.language | eng | |
dc.publisher | MDPI | en_US |
dc.relation.ispartof | Antibodies (Basel) | |
dc.rights | This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). | |
dc.subject | IgD | en_US |
dc.subject | IgE | en_US |
dc.subject | Phl p 7 | en_US |
dc.subject | anti-idiotype | en_US |
dc.subject | antibody | en_US |
dc.subject | inhibitor | en_US |
dc.subject | isotype | en_US |
dc.subject | nanobody | en_US |
dc.title | Expanding the Anti-Phl p 7 Antibody Toolkit: An Anti-Idiotype Nanobody Inhibitor. | en_US |
dc.type | Article | en_US |
dc.rights.holder | © 2023 by the authors. Licensee MDPI, Basel, Switzerland. | |
dc.identifier.doi | 10.3390/antib12040075 | |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/37987253 | en_US |
pubs.issue | 4 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published online | en_US |
pubs.volume | 12 | en_US |
dcterms.dateAccepted | 2023-11-10 | |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |