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dc.contributor.authorKhateb, H
dc.contributor.authorHook, AL
dc.contributor.authorKern, S
dc.contributor.authorWatts, JA
dc.contributor.authorSingh, S
dc.contributor.authorJackson, D
dc.contributor.authorMarinez-Pomares, L
dc.contributor.authorWilliams, P
dc.contributor.authorAlexander, MR
dc.date.accessioned2024-05-02T16:07:58Z
dc.date.available2023-05-09
dc.date.available2024-05-02T16:07:58Z
dc.date.issued2023-05-31
dc.identifier.citationBiointerphases 18, 031007 (2023); doi: 10.1116/6.0002604en_US
dc.identifier.issn1934-8630
dc.identifier.otherARTN 031007
dc.identifier.urihttps://qmro.qmul.ac.uk/xmlui/handle/123456789/96629
dc.description.abstractSecondary ion mass spectrometry (SIMS) offers advantages over both liquid extraction mass spectrometry and matrix assisted laser desorption mass spectrometry in that it provides the direct in situ analysis of molecules and has the potential to preserve the 3D location of an analyte in a sample. Polysaccharides are recognized as challenging analytes in the mass spectrometry of liquids and are also difficult to identify and assign using SIMS. Psl is an exopolysaccharide produced by Pseudomonas aeruginosa, which plays a key role in biofilm formation and maturation. In this Letter, we describe the use of the OrbiTrap analyzer with SIMS (3D OrbiSIMS) for the label-free mass spectrometry of Psl, taking advantage of its high mass resolving power for accurate secondary ion assignment. We study a P. aeruginosa biofilm and compare it with purified Psl to enable the assignment of secondary ions specific to the Psl structure. This resulted in the identification of 17 peaks that could confidently be ascribed to Psl fragments within the biofilm matrix. The complementary approach of the following neutral loss sequences is also shown to identify multiple oligosaccharide fragments without the requirement of a biological reference sample.en_US
dc.language.isoenen_US
dc.publisherAIP Publishingen_US
dc.relation.ispartofBiointerphases
dc.rightsAll article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). https://doi.org/10.1116/6.0002604
dc.titleIdentification of Pseudomonas aeruginosa exopolysaccharide Psl in biofilms using 3D OrbiSIMSen_US
dc.typeArticleen_US
dc.rights.holder© 2023 Author(s).
dc.identifier.doi10.1116/6.0002604
pubs.author-urlhttps://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:001000077500002&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=612ae0d773dcbdba3046f6df545e9f6aen_US
pubs.issue3en_US
pubs.notesNot knownen_US
pubs.publication-statusPublisheden_US
pubs.volume18en_US
rioxxterms.funderDefault funderen_US
rioxxterms.identifier.projectDefault projecten_US


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