Protection of photosystem I during sudden light stress depends on ferredoxin:NADP(H) reductase abundance and interactions
dc.contributor.author | Rodriguez-Heredia, M | |
dc.contributor.author | Saccon, F | |
dc.contributor.author | Wilson, S | |
dc.contributor.author | Finazzi, G | |
dc.contributor.author | Ruban, AV | |
dc.contributor.author | Hanke, GT | |
dc.date.accessioned | 2024-01-05T12:15:23Z | |
dc.date.available | 2021-10-29 | |
dc.date.available | 2024-01-05T12:15:23Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 0032-0889 | |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/93444 | |
dc.description.abstract | Plant tolerance to high light and oxidative stress is increased by overexpression of the photosynthetic enzyme Ferredoxin:NADP(H) reductase (FNR), but the specific mechanism of FNR-mediated protection remains enigmatic. It has also been reported that the localization of this enzyme within the chloroplast is related to its role in stress tolerance. Here, we dissected the impact of FNR content and location on photoinactivation of photosystem I (PSI) and photosystem II (PSII) during high light stress of Arabidopsis (Arabidopsis thaliana). The reaction center of PSII is efficiently turned over during light stress, while damage to PSI takes much longer to repair. Our results indicate a PSI sepcific effect, where efficient oxidation of the PSI primary donor (P700) upon transition from darkness to light, depends on FNR recruitment to the thylakoid membrane tether proteins: thylakoid rhodanase-like protein (TROL) and translocon at the inner envelope of chloroplasts 62 (Tic62). When these interactions were disrupted, PSI photoinactivation occurred. In contrast, there was a moderate delay in the onset of PSII damage. Based on measurements of ΔpH formation and cyclic electron flow, we propose that FNR location influences the speed at which photosynthetic control is induced, resulting in specific impact on PSI damage. Membrane tethering of FNR therefore plays a role in alleviating high light stress, by regulating electron distribution during short-term responses to light. | en_US |
dc.format.extent | 1028 - 1042 | |
dc.publisher | Oxford University Press | en_US |
dc.relation.ispartof | PLANT PHYSIOLOGY | |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | |
dc.rights | Attribution 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
dc.title | Protection of photosystem I during sudden light stress depends on ferredoxin:NADP(H) reductase abundance and interactions | en_US |
dc.type | Article | en_US |
dc.rights.holder | © The Author(s) 2021. Published by Oxford University Press on behalf of American Society of Plant Biologists. | |
dc.identifier.doi | 10.1093/plphys/kiab550 | |
pubs.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000761476800035&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=612ae0d773dcbdba3046f6df545e9f6a | en_US |
pubs.issue | 2 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 188 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |
qmul.funder | Novel methodology for quantitative assessment of the capacity for photoprotection in photosynthetic organisms::BBSRC | en_US |
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