dc.contributor.author | Penkava, F | en_US |
dc.contributor.author | Velasco-Herrera, MDC | en_US |
dc.contributor.author | Young, MD | en_US |
dc.contributor.author | Yager, N | en_US |
dc.contributor.author | Nwosu, LN | en_US |
dc.contributor.author | Pratt, AG | en_US |
dc.contributor.author | Lara, AL | en_US |
dc.contributor.author | Guzzo, C | en_US |
dc.contributor.author | Maroof, A | en_US |
dc.contributor.author | Mamanova, L | en_US |
dc.contributor.author | Cole, S | en_US |
dc.contributor.author | Efremova, M | en_US |
dc.contributor.author | Simone, D | en_US |
dc.contributor.author | Filer, A | en_US |
dc.contributor.author | Brown, CC | en_US |
dc.contributor.author | Croxford, AL | en_US |
dc.contributor.author | Isaacs, JD | en_US |
dc.contributor.author | Teichmann, S | en_US |
dc.contributor.author | Bowness, P | en_US |
dc.contributor.author | Behjati, S | en_US |
dc.contributor.author | Hussein Al-Mossawi, M | en_US |
dc.date.accessioned | 2020-10-20T16:06:45Z | |
dc.date.available | 2020-08-27 | en_US |
dc.date.issued | 2020-09-21 | en_US |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/67651 | |
dc.description.abstract | Psoriatic arthritis (PsA) is a debilitating immune-mediated inflammatory arthritis of unknown pathogenesis commonly affecting patients with skin psoriasis. Here we use complementary single-cell approaches to study leukocytes from PsA joints. Mass cytometry demonstrates a 3-fold expansion of memory CD8 T cells in the joints of PsA patients compared to peripheral blood. Meanwhile, droplet-based and plate-based single-cell RNA sequencing of paired T cell receptor alpha and beta chain sequences show pronounced CD8 T cell clonal expansions within the joints. Transcriptome analyses find these expanded synovial CD8 T cells to express cycling, activation, tissue-homing and tissue residency markers. T cell receptor sequence comparison between patients identifies clonal convergence. Finally, chemokine receptor CXCR3 is upregulated in the expanded synovial CD8 T cells, while two CXCR3 ligands, CXCL9 and CXCL10, are elevated in PsA synovial fluid. Our data thus provide a quantitative molecular insight into the cellular immune landscape of psoriatic arthritis. | en_US |
dc.format.extent | 4767 - ? | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Nat Commun | en_US |
dc.rights | Creative Commons Attribution 4.0 International License | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Arthritis, Psoriatic | en_US |
dc.subject | CD8-Positive T-Lymphocytes | en_US |
dc.subject | Clonal Selection, Antigen-Mediated | en_US |
dc.subject | Gene Expression Profiling | en_US |
dc.subject | Humans | en_US |
dc.subject | Immunologic Memory | en_US |
dc.subject | Receptors, Antigen, T-Cell, alpha-beta | en_US |
dc.subject | Receptors, Chemokine | en_US |
dc.subject | Receptors, Lymphocyte Homing | en_US |
dc.subject | Single-Cell Analysis | en_US |
dc.subject | Synovial Fluid | en_US |
dc.subject | Synovial Membrane | en_US |
dc.title | Single-cell sequencing reveals clonal expansions of pro-inflammatory synovial CD8 T cells expressing tissue-homing receptors in psoriatic arthritis. | en_US |
dc.type | Article | |
dc.rights.holder | © The Author(s) 2020 | |
dc.identifier.doi | 10.1038/s41467-020-18513-6 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/32958743 | en_US |
pubs.issue | 1 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published online | en_US |
pubs.volume | 11 | en_US |
dcterms.dateAccepted | 2020-08-27 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |