dc.contributor.author | Robertson, KA | |
dc.contributor.author | Hsieh, WY | |
dc.contributor.author | Forster, T | |
dc.contributor.author | Blanc, M | |
dc.contributor.author | Lu, H | |
dc.contributor.author | Crick, PJ | |
dc.contributor.author | Yutuc, E | |
dc.contributor.author | Watterson, S | |
dc.contributor.author | Martin, K | |
dc.contributor.author | Griffiths, SJ | |
dc.contributor.author | Enright, AJ | |
dc.contributor.author | Yamamoto, M | |
dc.contributor.author | Pradeepa, MM | |
dc.contributor.author | Lennox, KA | |
dc.contributor.author | Behlke, MA | |
dc.contributor.author | Talbot, S | |
dc.contributor.author | Haas, J | |
dc.contributor.author | Doelken, L | |
dc.contributor.author | Griffiths, WJ | |
dc.contributor.author | Wang, Y | |
dc.contributor.author | Angulo, A | |
dc.contributor.author | Ghazal, P | |
dc.date.accessioned | 2019-03-27T12:49:11Z | |
dc.date.available | 2015-12-22 | |
dc.date.available | 2019-03-27T12:49:11Z | |
dc.date.issued | 2016-03-03 | |
dc.identifier.citation | Robertson KA, Hsieh WY, Forster T, Blanc M, Lu H, Crick PJ, et al. (2016) An Interferon Regulated MicroRNA Provides Broad Cell-Intrinsic Antiviral Immunity through Multihit Host-Directed Targeting of the Sterol Pathway. PLoS Biol 14(3): e1002364. https://doi.org/10.1371/journal.pbio.1002364 | en_US |
dc.identifier.issn | 1545-7885 | |
dc.identifier.other | ARTN e1002364 | |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/56515 | |
dc.description.abstract | In invertebrates, small interfering RNAs are at the vanguard of cell-autonomous antiviral immunity. In contrast, antiviral mechanisms initiated by interferon (IFN) signaling predominate in mammals. Whilst mammalian IFN-induced miRNA are known to inhibit specific viruses, it is not known whether host-directed microRNAs, downstream of IFN-signaling, have a role in mediating broad antiviral resistance. By performing an integrative, systematic, global analysis of RNA turnover utilizing 4-thiouridine labeling of newly transcribed RNA and pri/pre-miRNA in IFN-activated macrophages, we identify a new post-transcriptional viral defense mechanism mediated by miR-342-5p. On the basis of ChIP and site-directed promoter mutagenesis experiments, we find the synthesis of miR-342-5p is coupled to the antiviral IFN response via the IFN-induced transcription factor, IRF1. Strikingly, we find miR-342-5p targets mevalonate-sterol biosynthesis using a multihit mechanism suppressing the pathway at different functional levels: transcriptionally via SREBF2, post-transcriptionally via miR-33, and enzymatically via IDI1 and SC4MOL. Mass spectrometry-based lipidomics and enzymatic assays demonstrate the targeting mechanisms reduce intermediate sterol pathway metabolites and total cholesterol in macrophages. These results reveal a previously unrecognized mechanism by which IFN regulates the sterol pathway. The sterol pathway is known to be an integral part of the macrophage IFN antiviral response, and we show that miR-342-5p exerts broad antiviral effects against multiple, unrelated pathogenic viruses such Cytomegalovirus and Influenza A (H1N1). Metabolic rescue experiments confirm the specificity of these effects and demonstrate that unrelated viruses have differential mevalonate and sterol pathway requirements for their replication. This study, therefore, advances the general concept of broad antiviral defense through multihit targeting of a single host pathway.
Author Summary
How infected cells respond to a virus during the first minutes to hours after infection can determine whether a disease develops and influences the host’s long-term survival. In mammals, unlike plants and flies that use small RNAs to fight viral infections, virus-induced interferon responses are a critical early event resulting in broad protection against infection. Interferon is a secreted host protein that binds to receptors on the surface of infected and uninfected cells and activates biochemical pathways that profoundly change the expression of hundreds of cellular genes, including those encoding microRNAs. The antiviral functions of only a handful of these genes are understood, and it is not known how the majority contribute to broadly protect against many different viruses. In this study, we uncover an interferon-regulated microRNA (miR-342-5p) that contributes to broad host cell immunity against infection through the cholesterol biosynthesis pathway. We show that miR-342-5p does this through a multihit strategy, turning down the master regulator of sterol biosynthesis as well as several specifically targeted enzymes within the pathway. A wide range of viruses depend on a number of the metabolite side-branches of the sterol biosynthesis pathway for their replication. Notably, our study reveals that by utilising multihit targeting of key branch-points in a single pathway, miR-342-5p is able to inhibit the replication of unrelated, clinically significant pathogens ranging from Herpes to Flu viruses. | en_US |
dc.language.iso | en | en_US |
dc.publisher | Public Library of Science | en_US |
dc.relation.ispartof | PLOS BIOLOGY | |
dc.rights | Creative Commons Attribution License | |
dc.rights | Attribution 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
dc.subject | Interferon | en_US |
dc.subject | MicroRNA | en_US |
dc.subject | Antiviral Immunity | en_US |
dc.subject | sterol biosynthesis pathways | en_US |
dc.title | An Interferon Regulated MicroRNA Provides Broad Cell-Intrinsic Antiviral Immunity through Multihit Host-Directed Targeting of the Sterol Pathway | en_US |
dc.type | Article | en_US |
dc.rights.holder | 2016 Robertson et al. | |
dc.identifier.doi | 10.1371/journal.pbio.1002364 | |
pubs.author-url | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000373038200003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=612ae0d773dcbdba3046f6df545e9f6a | en_US |
pubs.issue | 3 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 14 | en_US |
dcterms.dateAccepted | 2015-12-22 | |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |