Novel methods in adrenal research: a metabolomics approach.
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Publisher
DOI
10.1007/s00418-019-01772-w
Journal
Histochem Cell Biol
ISSN
0948-6143
Metadata
Show full item recordAbstract
Metabolic alterations have implications in a spectrum of tissue functions and disease. Aided by novel molecular biological and computational tools, our understanding of physiological and pathological processes underpinning endocrine and endocrine-related disease has significantly expanded over the last decade. Herein, we focus on novel metabolomics-related methodologies in adrenal research: in situ metabolomics by mass spectrometry imaging, steroid metabolomics by gas and liquid chromatography-mass spectrometry, energy pathway metabologenomics by liquid chromatography-mass spectrometry-based metabolomics of Krebs cycle intermediates, and cellular reprogramming to generate functional steroidogenic cells and hence to modulate the steroid metabolome. All four techniques to assess and/or modulate the metabolome in biological systems provide tremendous opportunities to manage neoplastic and non-neoplastic disease of the adrenal glands in the era of precision medicine. In this context, we discuss emerging clinical applications and/or promising metabolic-driven research towards diagnostic, prognostic, predictive and therapeutic biomarkers in tumours arising from the adrenal gland and extra-adrenal paraganglia as well as modern approaches to delineate and reprogram adrenal metabolism.
Authors
Papathomas, TG; Sun, N; Chortis, V; Taylor, AE; Arlt, W; Richter, S; Eisenhofer, G; Ruiz-Babot, G; Guasti, L; Walch, AKCollections
- Centre for Endocrinology [552]