dc.contributor.author | Worrall, JT | en_US |
dc.contributor.author | Tamura, N | en_US |
dc.contributor.author | Mazzagatti, A | en_US |
dc.contributor.author | Shaikh, N | en_US |
dc.contributor.author | van Lingen, T | en_US |
dc.contributor.author | Bakker, B | en_US |
dc.contributor.author | Spierings, DCJ | en_US |
dc.contributor.author | Vladimirou, E | en_US |
dc.contributor.author | Foijer, F | en_US |
dc.contributor.author | McClelland, SE | en_US |
dc.date.accessioned | 2018-12-17T14:22:45Z | |
dc.date.available | 2018-05-14 | en_US |
dc.date.issued | 2018-06-12 | en_US |
dc.date.submitted | 2018-12-07T11:05:18.428Z | |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/53803 | |
dc.description.abstract | A common assumption is that human chromosomes carry equal chances of mis-segregation during compromised cell division. Human chromosomes vary in multiple parameters that might generate bias, but technological limitations have precluded a comprehensive analysis of chromosome-specific aneuploidy. Here, by imaging specific centromeres coupled with high-throughput single-cell analysis as well as single-cell sequencing, we show that aneuploidy occurs non-randomly following common treatments to elevate chromosome mis-segregation. Temporary spindle disruption leads to elevated mis-segregation and aneuploidy of a subset of chromosomes, particularly affecting chromosomes 1 and 2. Unexpectedly, we find that a period of mitotic delay weakens centromeric cohesion and promotes chromosome mis-segregation and that chromosomes 1 and 2 are particularly prone to suffer cohesion fatigue. Our findings demonstrate that inherent properties of individual chromosomes can bias chromosome mis-segregation and aneuploidy rates, with implications for studies on aneuploidy in human disease. | en_US |
dc.format.extent | 3366 - 3380 | en_US |
dc.language | eng | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Cell Rep | en_US |
dc.rights | This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). | |
dc.subject | ImageStream | en_US |
dc.subject | aneuploidy | en_US |
dc.subject | chromosome mis-segregation | en_US |
dc.subject | cohesion fatigue | en_US |
dc.title | Non-random Mis-segregation of Human Chromosomes. | en_US |
dc.type | Article | |
dc.rights.holder | (c) 2018 The Author(s) | |
dc.identifier.doi | 10.1016/j.celrep.2018.05.047 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/29898405 | en_US |
pubs.issue | 11 | en_US |
pubs.notes | No embargo | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 23 | en_US |
dcterms.dateAccepted | 2018-05-14 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |
rioxxterms.licenseref.uri | https://creativecommons.org/licenses/by/4.0/ | en_US |