dc.contributor.author | Danson, AF | en_US |
dc.contributor.author | Marzi, SJ | en_US |
dc.contributor.author | Lowe, R | en_US |
dc.contributor.author | Holland, ML | en_US |
dc.contributor.author | Rakyan, VK | en_US |
dc.date.accessioned | 2018-05-03T15:35:44Z | |
dc.date.available | 2018-04-09 | en_US |
dc.date.issued | 2018-05-02 | en_US |
dc.date.submitted | 2018-05-03T15:51:04.558Z | |
dc.identifier.other | 10.1186/s12915-018-0516-5 | |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/36711 | |
dc.description | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made | en_US |
dc.description.abstract | BACKGROUND: Environmental influences fluctuate throughout the life course of an organism. It is therefore important to understand how the timing of exposure impacts molecular responses. Herein, we examine the responses of two key molecular markers of dietary stress, namely variant-specific methylation at ribosomal DNA (rDNA) and small RNA distribution, including tRNA fragments, in a mouse model of protein restriction (PR) with exposure at pre- and/or post-weaning. RESULTS: We first confirm that pre-weaning PR exposure modulates the methylation state of rDNA in a genotype-dependent manner, whereas post-weaning PR exposure has no such effect. Conversely, post-weaning PR induces a shift in small RNA distribution, but there is no effect in the pre-weaning PR model. Intriguingly, mice exposed to PR throughout their lives show neither of these two dietary stress markers, similar to controls. CONCLUSIONS: The results show that the timing of the insult affects the nature of the molecular response but also, critically, that 'matching' diet exposure either side of weaning eliminates the stress response at the level of rDNA methylation and small RNA in sperm. | en_US |
dc.description.sponsorship | AFD is funded by an MRC Studentship (MR/K501372/1) and a Life Sciences Initiative Small Grant and the work was supported by a Biotechnology and Biological Sciences Research Council, UK (BB/M012494/1) grant awarded to VKR. | en_US |
dc.format.extent | 51 - ? | en_US |
dc.language | eng | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | BMC Biol | en_US |
dc.rights | CC BY | |
dc.subject | DNA methylation | en_US |
dc.subject | Nutrition | en_US |
dc.subject | mismatch | en_US |
dc.subject | protein restriction | en_US |
dc.subject | ribosomal DNA | en_US |
dc.subject | small RNA | en_US |
dc.subject | Animals | en_US |
dc.subject | DNA Methylation | en_US |
dc.subject | DNA, Ribosomal | en_US |
dc.subject | Diet, Protein-Restricted | en_US |
dc.subject | Female | en_US |
dc.subject | Male | en_US |
dc.subject | Mice | en_US |
dc.subject | Weaning | en_US |
dc.title | Early life diet conditions the molecular response to post-weaning protein restriction in the mouse. | en_US |
dc.type | Article | |
dc.rights.holder | Rakyan et al. 2018 | |
dc.identifier.doi | 10.1186/s12915-018-0516-5 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/29720174 | en_US |
pubs.issue | 1 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published online | en_US |
pubs.volume | 16 | en_US |
dcterms.dateAccepted | 2018-04-09 | en_US |