dc.contributor.author | Ghayad, SE | en_US |
dc.contributor.author | Rammal, G | en_US |
dc.contributor.author | Ghamloush, F | en_US |
dc.contributor.author | Basma, H | en_US |
dc.contributor.author | Nasr, R | en_US |
dc.contributor.author | Diab-Assaf, M | en_US |
dc.contributor.author | Chelala, C | en_US |
dc.contributor.author | Saab, R | en_US |
dc.date.accessioned | 2018-01-22T10:47:17Z | |
dc.date.available | 2016-10-21 | en_US |
dc.date.issued | 2016-11-17 | en_US |
dc.date.submitted | 2018-01-17T21:23:59.637Z | |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/31466 | |
dc.description.abstract | Rhabdomyosarcoma (RMS) is an aggressive childhood soft tissue tumor, which exists in oncoprotein PAX-FOXO1 fusion positive and fusion negative subtypes, with the fusion-positive RMS being characterized by a more aggressive clinical behavior. Exosomes are small membranous vesicles secreted into body fluids by multiple cell types, including tumor cells, and have been implicated in metastatic progression through paracrine signaling. We characterized exosomes secreted by a panel of 5 RMS cell lines. Expression array analysis showed that, for both fusion-positive and fusion-negative cells, exosome miRNA clustered well together and to a higher extent than cellular miRNA. While enriched miRNA in exosomes of fusion-negative RMS cells were distinct from those of fusion-positive RMS cells, the most significant predicted disease and functions in both groups were related to processes relevant to cancer and tissue remodelling. Functionally, we found that RMS-derived exosomes exerted a positive effect on cellular proliferation of recipient RMS cells and fibroblasts, induced cellular migration and invasion of fibroblasts, and promoted angiogenesis. These findings show that RMS-derived exosomes enhance invasive properties of recipient cells, and that exosome content of fusion-positive RMS is different than that of fusion-negative RMS, possibly contributing to the different metastatic propensity of the two subtypes. | en_US |
dc.description.sponsorship | MPP grant from the American University of Beirut Medical Center, Faculty of Medicine and with support
from the Lebanese University. Miss Ghina Rammal has PhD funding by Azm and Saade association. The Saab
laboratory was also supported in part by the International Outreach Program at St Jude Children’s Research
Hospital and the American Lebanese Syrian Associated Charities (ALSAC), Memphis, TN, and the Children’s
Cancer Center of Lebanon in Beirut, Lebanon. | en_US |
dc.format.extent | 37088 - ? | en_US |
dc.language | eng | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Sci Rep | en_US |
dc.rights | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | |
dc.subject | Animals | en_US |
dc.subject | Cell Line, Tumor | en_US |
dc.subject | Cell Proliferation | en_US |
dc.subject | Cell-Derived Microparticles | en_US |
dc.subject | Fibroblasts | en_US |
dc.subject | Human Umbilical Vein Endothelial Cells | en_US |
dc.subject | Humans | en_US |
dc.subject | Mice | en_US |
dc.subject | MicroRNAs | en_US |
dc.subject | Paracrine Communication | en_US |
dc.subject | RNA, Neoplasm | en_US |
dc.subject | Rhabdomyosarcoma, Alveolar | en_US |
dc.subject | Rhabdomyosarcoma, Embryonal | en_US |
dc.title | Exosomes derived from embryonal and alveolar rhabdomyosarcoma carry differential miRNA cargo and promote invasion of recipient fibroblasts. | en_US |
dc.type | Article | |
dc.rights.holder | © The Author(s) 2016 | |
dc.identifier.doi | 10.1038/srep37088 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/27853183 | en_US |
pubs.notes | No embargo | en_US |
pubs.publication-status | Published online | en_US |
pubs.volume | 6 | en_US |
dcterms.dateAccepted | 2016-10-21 | en_US |