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dc.contributor.authorHirth, M
dc.contributor.authorLiverani, S
dc.contributor.authorMahlow, S
dc.contributor.authorBouget, F-Y
dc.contributor.authorPohnert, G
dc.contributor.authorSasso, S
dc.date.accessioned2017-10-24T09:03:57Z
dc.date.available2017-10-24T09:03:57Z
dc.date.issued2017-04
dc.date.submitted2017-10-11T16:29:37.553Z
dc.identifier.citationHirth, M., Liverani, S., Mahlow, S., Bouget, F., Pohnert, G. and Sasso, S. (2017). Metabolic profiling identifies trehalose as an abundant and diurnally fluctuating metabolite in the microalga Ostreococcus tauri. [online] Metabolomics. Available at: https://link.springer.com/article/10.1007%2Fs11306-017-1203-1 [Accessed 24 Oct. 2017].en_US
dc.identifier.issn1573-3882
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/28304
dc.description.abstractINTRODUCTION: The picoeukaryotic alga Ostreococcus tauri (Chlorophyta) belongs to the widespread group of marine prasinophytes. Despite its ecological importance, little is known about the metabolism of this alga. OBJECTIVES: In this work, changes in the metabolome were quantified when O. tauri was grown under alternating cycles of 12 h light and 12 h darkness. METHODS: Algal metabolism was analyzed by gas chromatography-mass spectrometry. Using fluorescence-activated cell sorting, the bacteria associated with O. tauri were depleted to below 0.1% of total cells at the time of metabolic profiling. RESULTS: Of 111 metabolites quantified over light-dark cycles, 20 (18%) showed clear diurnal variations. The strongest fluctuations were found for trehalose. With an intracellular concentration of 1.6 mM in the dark, this disaccharide was six times more abundant at night than during the day. This fluctuation pattern of trehalose may be a consequence of starch degradation or of the synchronized cell cycle. On the other hand, maltose (and also sucrose) was below the detection limit (~10 μM). Accumulation of glycine in the light is in agreement with the presence of a classical glycolate pathway of photorespiration. We also provide evidence for the presence of fatty acid methyl and ethyl esters in O. tauri. CONCLUSIONS: This study shows how the metabolism of O. tauri adapts to day and night and gives new insights into the configuration of the carbon metabolism. In addition, several less common metabolites were identified.en_US
dc.description.sponsorshipM.H. and S.S. acknowledge financial support by the Jena School for Microbial Communication (GSC 214/2), which is funded by the German Research Foundation (DFG). G.P. and S.S. are supported by the DFG-funded Collaborative Research Centre ChemBioSys (SFB 1127).en_US
dc.format.extent68 - ?
dc.languageeng
dc.publisherSpringeren_US
dc.relation.ispartofMetabolomics
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
dc.subjectCarbon metabolismen_US
dc.subjectMicroalgaeen_US
dc.subjectPicoeukaryotesen_US
dc.subjectUntargeted metabolomicsen_US
dc.subjectpantothenate.en_US
dc.titleMetabolic profiling identifies trehalose as an abundant and diurnally fluctuating metabolite in the microalga Ostreococcus tauri.en_US
dc.typeArticleen_US
dc.rights.holder© The Author(s) 2017
dc.identifier.doi10.1007/s11306-017-1203-1
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/28473745
pubs.issue6
pubs.organisational-group/Queen Mary University of London
pubs.organisational-group/Queen Mary University of London/Faculty of Science & Engineering
pubs.organisational-group/Queen Mary University of London/Faculty of Science & Engineering/Mathematical Sciences - Staff and Research Students
pubs.publication-statusPublished
pubs.volume13


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