dc.contributor.author | Glyde, R | en_US |
dc.contributor.author | Ye, F | en_US |
dc.contributor.author | Darbari, VC | en_US |
dc.contributor.author | Zhang, N | en_US |
dc.contributor.author | Buck, M | en_US |
dc.contributor.author | Zhang, X | en_US |
dc.date.accessioned | 2017-06-08T14:15:43Z | |
dc.date.available | 2017-05-05 | en_US |
dc.date.issued | 2017-07-06 | en_US |
dc.date.submitted | 2017-06-05T19:41:21.360Z | |
dc.identifier.uri | http://qmro.qmul.ac.uk/xmlui/handle/123456789/23687 | |
dc.description.abstract | Gene transcription is carried out by RNA polymerases (RNAPs). For transcription to occur, the closed promoter complex (RPc), where DNA is double stranded, must isomerize into an open promoter complex (RPo), where the DNA is melted out into a transcription bubble and the single-stranded template DNA is delivered to the RNAP active site. Using a bacterial RNAP containing the alternative σ54 factor and cryoelectron microscopy, we determined structures of RPc and the activator-bound intermediate complex en route to RPo at 3.8 and 5.8 Å. Our structures show how RNAP-σ54 interacts with promoter DNA to initiate the DNA distortions required for transcription bubble formation, and how the activator interacts with RPc, leading to significant conformational changes in RNAP and σ54 that promote RPo formation. We propose that DNA melting is an active process initiated in RPc and that the RNAP conformations of intermediates are significantly different from that of RPc and RPo. | en_US |
dc.format.extent | 106 - 116.e4 | en_US |
dc.language | eng | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Mol Cell | en_US |
dc.rights | CC-BY | |
dc.subject | AAA protein | en_US |
dc.subject | DNA distortion | en_US |
dc.subject | DNA opening | en_US |
dc.subject | RNA polymerase | en_US |
dc.subject | sigma factor | en_US |
dc.subject | sigma54 | en_US |
dc.subject | transcription bubble | en_US |
dc.subject | transcription closed complex | en_US |
dc.subject | transcription initiation | en_US |
dc.subject | transcription intermediate complex | en_US |
dc.subject | Binding Sites | en_US |
dc.subject | Cryoelectron Microscopy | en_US |
dc.subject | DNA, Single-Stranded | en_US |
dc.subject | Escherichia coli | en_US |
dc.subject | Gene Expression Regulation, Bacterial | en_US |
dc.subject | Klebsiella pneumoniae | en_US |
dc.subject | Molecular Docking Simulation | en_US |
dc.subject | Nucleic Acid Conformation | en_US |
dc.subject | Nucleic Acid Denaturation | en_US |
dc.subject | Promoter Regions, Genetic | en_US |
dc.subject | Protein Binding | en_US |
dc.subject | Protein Conformation | en_US |
dc.subject | RNA Polymerase Sigma 54 | en_US |
dc.subject | Structure-Activity Relationship | en_US |
dc.subject | Transcription Initiation, Genetic | en_US |
dc.title | Structures of RNA Polymerase Closed and Intermediate Complexes Reveal Mechanisms of DNA Opening and Transcription Initiation. | en_US |
dc.type | Article | |
dc.rights.holder | © 2017 The Author(s). | |
dc.identifier.doi | 10.1016/j.molcel.2017.05.010 | en_US |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/28579332 | en_US |
pubs.issue | 1 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 67 | en_US |
dcterms.dateAccepted | 2017-05-05 | en_US |