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    Profiling DNA Methylation and Hydroxymethylation at Retrotransposable Elements. 
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    • Profiling DNA Methylation and Hydroxymethylation at Retrotransposable Elements.
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    Profiling DNA Methylation and Hydroxymethylation at Retrotransposable Elements.

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    Accepted version
    Embargoed until: 2100-01-01
    Volume
    1400
    Pagination
    387 - 401
    DOI
    10.1007/978-1-4939-3372-3_24
    Journal
    Methods Mol Biol
    Metadata
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    Abstract
    DNA methylation is a key epigenetic modification controlling the transcriptional activity of mammalian retrotransposable elements. Its oxidation to DNA hydroxymethylation has been linked to DNA demethylation and reactivation of retrotransposons. Here we describe in detail protocols for three methods to measure DNA methylation and hydroxymethylation at specific genomic targets: glucMS-qPCR, and two sequencing approaches (pyrosequencing and high-throughput sequencing) for analyzing bisulfite- and oxidative bisulfite-modified DNA. All three techniques provide absolute measurements of methylation and hydroxymethylation levels at single-base resolution. Differences between the methods are discussed, mainly with respect to throughput and target coverage. These constitute the core techniques that are used in our laboratory for accurately surveying the epigenetics of retrotransposable elements.
    Authors
    de la Rica, L; Stanley, JS; Branco, MR
    URI
    http://qmro.qmul.ac.uk/xmlui/handle/123456789/12732
    Collections
    • Centre for Genomics and Child Health [683]
    Language
    eng
    Licence information
    The final publication is available at http://link.springer.com/protocol/10.1007/978-1-4939-3372-3_24
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