Browsing Library Administration by Author "Bradley, M"
Now showing items 1-20 of 27
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Bioorthogonal Swarming: In Situ Generation of Dendrimers
Zhang, Y; Ucuncu, M; Gambardella, A; Baibek, A; Geng, J; Zhang, S; Clavadetscher, J; Litzen, I; Bradley, M; Lilienkampf, A (American Chemical Society, 2020-12-16)With the aid of bioorthogonal chemistry, we demonstrate the fabrication of synthetic dendrimers in situ around living cells. Using tetrazine dienophile and aminooxyl/hydrazide aldehyde chemistries, the density of functional ... -
Combinatorial ECM Arrays Identify Cooperative Roles for Matricellular Proteins in Enhancing the Generation of TH+ Neurons From Human Pluripotent Cells.
Ahmed, M; Owens, MJS; Toledo, EM; Arenas, E; Bradley, M; Ffrench-Constant, C (2021)The development of efficient cell culture strategies for the generation of dopaminergic neurons is an important goal for transplantation-based approaches to treat Parkinson's disease. To identify extracellular matrix ... -
Controlled Intracellular Polymerization for Cancer Treatment
Zhang, Y; Gao, Q; Li, W; He, R; Zhu, L; Lian, Q; Wang, L; Li, Y; Bradley, M; Geng, J (2022) -
Design of Photosensitizing Agents for Targeted Antimicrobial Photodynamic Therapy.
Klausen, M; Ucuncu, M; Bradley, M (2020-11-10)Photodynamic inactivation of microorganisms has gained substantial attention due to its unique mode of action, in which pathogens are unable to generate resistance, and due to the fact that it can be applied in a minimally ... -
Dyeing fungi: amphotericin B based fluorescent probes for multiplexed imaging.
Baibek, A; Üçüncü, M; Short, B; Ramage, G; Lilienkampf, A; Bradley, M (2021-02-23)The clinically used antifungal polyene amphotericin B was conjugated, via the mycosamine and the aglycon moieties, to fluorophores. The Cy5 conjugated probe showed selective labelling of fungi in the presence of bacteria, ... -
A fluorogenic peptide-based smartprobe for the detection of neutrophil extracellular traps and inflammation
Rios, MR; Garoffolo, G; Rinaldi, G; Megia-Fernandez, A; Ferrari, S; Robb, CT; Rossi, AG; Pesce, M; Bradley, M (2021) -
A fluorogenic, peptide-based probe for the detection of Cathepsin D in macrophages.
Rodriguez-Rios, M; McHugh, BJ; Liang, Z; Megia-Fernandez, A; Lilienkampf, A; Dockrell, D; Bradley, M (2023-11-02)Cathepsin D is a protease that is an effector in the immune response of macrophages, yet to date, only a limited number of probes have been developed for its detection. Herein, we report a water soluble, highly sensitive, ... -
Fluorophore-Tagged Poly-Lysine RAFT Agents: Controlled Synthesis of Trackable Cell-Penetrating Peptide-Polymers.
Shaw, PA; Klausen, M; Lilienkampf, A; Bradley, M (2023-09-11)The conjugation of a fluorophore and a variety of cell-penetrating peptides onto a RAFT agent allowed for the synthesis of polymers of defined sizes with quantifiable cell-uptake. Each peptide-RAFT agent was used to ... -
Full spectrum fluorescence lifetime imaging with 0.5 nm spectral and 50 ps temporal resolution.
Williams, GOS; Williams, E; Finlayson, N; Erdogan, AT; Wang, Q; Fernandes, S; Akram, AR; Dhaliwal, K; Henderson, RK; Girkin, JM (2021-11-16)The use of optical techniques to interrogate wide ranging samples from semiconductors to biological tissue for rapid analysis and diagnostics has gained wide adoption over the past decades. The desire to collect ever more ... -
Hydrogel-based standards for single and multiphoton imaging at depth
Haseeb, F; Bourdakos, KN; Forsyth, E; Setchfield, K; Gorman, A; Venkateswaran, S; Wright, AJ; Mahajan, S; Bradley, M; Design and Quality for Biomedical Technologies XVII (SPIE, the international society for optics and photonics, 2024-03-13) -
Long-term feeder-free culture of human pancreatic progenitors on fibronectin or matrix-free polymer potentiates β cell differentiation.
Nakamura, A; Wong, YF; Venturato, A; Michaut, M; Venkateswaran, S; Santra, M; Gonçalves, C; Larsen, M; Leuschner, M; Kim, YH (2022-05-10)With the aim of producing β cells for replacement therapies to treat diabetes, several protocols have been developed to differentiate human pluripotent stem cells to β cells via pancreatic progenitors. While in vivo ... -
Moving into the red - a near infra-red optical probe for analysis of human neutrophil elastase in activated neutrophils and neutrophil extracellular traps.
Rodriguez-Rios, M; Rinaldi, G; Megia-Fernandez, A; Lilienkampf, A; Robb, CT; Rossi, AG; Bradley, M (2023-09-11)Neutrophils are the first immune cells recruited for defence against invading pathogens; however, their dysregulated activation and subsequent release of the enzyme human neutrophil elastase is associated with several, ... -
Near-Infrared-Emitting Hemicyanines and Their Photodynamic Killing of Cancer Cells
Santra, M; Owens, M; Birch, G; Bradley, M (2021) -
A niche-mimicking polymer hydrogel-based approach to identify molecular targets for tackling human pancreatic cancer stem cells.
Murota, Y; Nagane, M; Wu, M; Santra, M; Venkateswaran, S; Tanaka, S; Bradley, M; Taga, T; Tabu, K (2023-09-27)BACKGROUND: Pancreatic adenocarcinoma (PAAD) is one of the most fatal human cancers, but effective therapies remain to be established. Cancer stem cells (CSCs) are highly resistant to anti-cancer drugs and a deeper ... -
Optical Detection of Distal Lung Enzyme Activity in Human Inflammatory Lung Disease.
Megia-Fernandez, A; Marshall, A; Akram, AR; Mills, B; Chankeshwara, SV; Scholefield, E; Miele, A; McGorum, BC; Michaels, C; Knighton, N (2021)Objective and Impact Statement. There is a need to develop platforms delineating inflammatory biology of the distal human lung. We describe a platform technology approach to detect in situ enzyme activity and observe drug ... -
Peptide probes for proteases - innovations and applications for monitoring proteolytic activity.
Rodriguez-Rios, M; Megia-Fernandez, A; Norman, DJ; Bradley, M (2022-03-21)Proteases are excellent biomarkers for a variety of diseases, offer multiple opportunities for diagnostic applications and are valuable targets for therapy. From a chemistry-based perspective this review discusses and ... -
Polymers showing intrinsic antimicrobial activity.
Haktaniyan, M; Bradley, M (2022-10-17)Pathogenic microorganisms are considered to a major threat to human health, impinging on multiple sectors including hospitals, dentistry, food storage and packaging, and water contamination. Due to the increasing levels ... -
Programmable and Flexible Fluorochromic Polymer Microarrays for Information Storage
Xia, H; Ding, Y; Gong, J; Lilienkampf, A; Xie, K; Bradley, M (2022) -
Rapid fabrication and screening of tailored functional 3D biomaterials: Validation in bone tissue repair - Part II.
Conde-González, A; Glinka, M; Dutta, D; Wallace, R; Callanan, A; Oreffo, ROC; Bradley, M (2023-02)Regenerative medicine strategies place increasingly sophisticated demands on 3D biomaterials to promote tissue formation at sites where tissue would otherwise not form. Ideally, the discovery/fabrication of the 3D scaffolds ... -
Self-healing multilayer films for simultaneous release of hydrophilic and hydrophobic drugs
Sun, YX; Wang, W; Sheng, Y; Zhang, R; Bradley, M (2021-01-01)Coloading and codelivery of hydrophilic and hydrophobic drugs as techniques for effective therapy have drawn attention but still present a challenge owing to the opposite nature of both drugs. Therefore, determining a ...