dc.contributor.author | Leckey, CA | |
dc.contributor.author | Coulton, JB | |
dc.contributor.author | Giovannucci, TA | |
dc.contributor.author | He, Y | |
dc.contributor.author | Aslanyan, A | |
dc.contributor.author | Laban, R | |
dc.contributor.author | Heslegrave, A | |
dc.contributor.author | Doykov, I | |
dc.contributor.author | Ammoscato, F | |
dc.contributor.author | Chataway, J | |
dc.contributor.author | De Angelis, F | |
dc.contributor.author | Gnanapavan, S | |
dc.contributor.author | Byrne, LM | |
dc.contributor.author | Schott, JM | |
dc.contributor.author | Wild, EJ | |
dc.contributor.author | Barthelémy, NR | |
dc.contributor.author | Zetterberg, H | |
dc.contributor.author | Wray, S | |
dc.contributor.author | Bateman, RJ | |
dc.contributor.author | Mills, K | |
dc.contributor.author | Paterson, RW | |
dc.date.accessioned | 2024-07-23T11:42:57Z | |
dc.date.available | 2024-04-16 | |
dc.date.available | 2024-07-23T11:42:57Z | |
dc.date.issued | 2024 | |
dc.identifier.citation | Claire A Leckey, John B Coulton, Tatiana A Giovannucci, Yingxin He, Aram Aslanyan, Rhiannon Laban, Amanda Heslegrave, Ivan Doykov, Francesca Ammoscato, Jeremy Chataway, Floriana De Angelis, Sharmilee Gnanapavan, Lauren M Byrne, Jonathan M Schott, Edward J Wild, Nicolas R Barthelémy, Henrik Zetterberg, Selina Wray, Randall J Bateman, Kevin Mills, Ross W Paterson, CSF neurofilament light chain profiling and quantitation in neurological diseases, Brain Communications, Volume 6, Issue 3, 2024, fcae132, https://doi.org/10.1093/braincomms/fcae132 | en_US |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/98352 | |
dc.description.abstract | Neurofilament light chain is an established marker of neuroaxonal injury that is elevated in CSF and blood across various neurological diseases. It is increasingly used in clinical practice to aid diagnosis and monitor progression and as an outcome measure to assess safety and efficacy of disease-modifying therapies across the clinical translational neuroscience field. Quantitative methods for neurofilament light chain in human biofluids have relied on immunoassays, which have limited capacity to describe the structure of the protein in CSF and how this might vary in different neurodegenerative diseases. In this study, we characterized and quantified neurofilament light chain species in CSF across neurodegenerative and neuroinflammatory diseases and healthy controls using targeted mass spectrometry. We show that the quantitative immunoprecipitation-tandem mass spectrometry method developed in this study strongly correlates to single-molecule array measurements in CSF across the broad spectrum of neurodegenerative diseases and was replicable across mass spectrometry methods and centres. In summary, we have created an accurate and cost-effective assay for measuring a key biomarker in translational neuroscience research and clinical practice, which can be easily multiplexed and translated into clinical laboratories for the screening and monitoring of neurodegenerative disease or acute brain injury. | en_US |
dc.format.extent | fcae132 - ? | |
dc.language | eng | |
dc.publisher | Oxford University Press | en_US |
dc.relation.ispartof | Brain Commun | |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | |
dc.subject | mass spectrometry | en_US |
dc.subject | neurodegenerative diseases | en_US |
dc.subject | neurofilament light chain | en_US |
dc.title | CSF neurofilament light chain profiling and quantitation in neurological diseases. | en_US |
dc.type | Article | en_US |
dc.rights.holder | © The Author(s) 2024. Published by Oxford University Press on behalf of the Guarantors of Brain. | |
dc.identifier.doi | 10.1093/braincomms/fcae132 | |
pubs.author-url | https://www.ncbi.nlm.nih.gov/pubmed/38707707 | en_US |
pubs.issue | 3 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published online | en_US |
pubs.volume | 6 | en_US |
dcterms.dateAccepted | 2024-04-12 | |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |
rioxxterms.funder.project | b215eee3-195d-4c4f-a85d-169a4331c138 | en_US |