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dc.contributor.authorThompson, CL
dc.contributor.authorChapple, JP
dc.contributor.authorKnight, MM
dc.date.accessioned2015-12-07T15:22:28Z
dc.date.issued2014-03
dc.date.issued2014-03
dc.date.issued2014-03
dc.date.issued2014-03
dc.date.issued2014-03
dc.date.issued2014-03
dc.identifier.urihttp://qmro.qmul.ac.uk/xmlui/handle/123456789/9635
dc.description.abstractOBJECTIVE: Hedgehog signalling is mediated by the primary cilium and promotes cartilage degeneration in osteoarthritis. Primary cilia are influenced by pathological stimuli and cilia length and prevalence are increased in osteoarthritic cartilage. This study aims to investigate the relationship between mechanical loading, hedgehog signalling and cilia disassembly in articular chondrocytes. METHODS: Primary bovine articular chondrocytes were subjected to cyclic tensile strain (CTS; 0.33 Hz, 10% or 20% strain). Hedgehog pathway activation (Ptch1, Gli1) and A Disintegrin And Metalloproteinase with Thrombospondin Motifs 5 (ADAMTS-5) expression were assessed by real-time PCR. A chondrocyte cell line generated from the Tg737(ORPK) mouse was used to investigate the role of the cilium in this response. Cilia length and prevalence were quantified by immunocytochemistry and confocal microscopy. RESULTS: Mechanical strain upregulates Indian hedgehog expression and activates hedgehog signalling. Ptch1, Gli1 and ADAMTS-5 expression were increased following 10% CTS, but not 20% CTS. Pathway activation requires a functioning primary cilium and is not observed in Tg737(ORPK) cells lacking cilia. Mechanical loading significantly reduced cilium length such that cilia became progressively shorter with increasing strain magnitude. Inhibition of histone deacetylase 6 (HDAC6), a tubulin deacetylase, prevented cilia disassembly and restored mechanosensitive hedgehog signalling and ADAMTS-5 expression at 20% CTS. CONCLUSIONS: This study demonstrates for the first time that mechanical loading activates primary cilia-mediated hedgehog signalling and ADAMTS-5 expression in adult articular chondrocytes, but that this response is lost at high strains due to HDAC6-mediated cilia disassembly. The study provides new mechanistic insight into the role of primary cilia and mechanical loading in articular cartilage.
dc.format.extent490 - 498
dc.languageeng
dc.relation.ispartofOsteoarthritis Cartilage
dc.rights10.1016/j.joca.2013.12.016
dc.subjectADAMTS-5
dc.subjectChondrocyte
dc.subjectCilia length
dc.subjectHedgehog
dc.subjectPrimary cilium
dc.subjectADAM Proteins
dc.subjectAnimals
dc.subjectCartilage, Articular
dc.subjectCattle
dc.subjectChondrocytes
dc.subjectCilia
dc.subjectHedgehog Proteins
dc.subjectHistone Deacetylases
dc.subjectMice
dc.subjectMicroscopy, Confocal
dc.subjectReal-Time Polymerase Chain Reaction
dc.subjectStress, Mechanical
dc.titlePrimary cilia disassembly down-regulates mechanosensitive hedgehog signalling: a feedback mechanism controlling ADAMTS-5 expression in chondrocytes.
dc.typeJournal Article
dc.identifier.doi10.1016/j.joca.2013.12.016
dc.relation.isPartOfOsteoarthritis Cartilage
dc.relation.isPartOfOsteoarthritis Cartilage
dc.relation.isPartOfOsteoarthritis Cartilage
pubs.author-urlhttps://www.ncbi.nlm.nih.gov/pubmed/24457103
pubs.issue3
pubs.organisational-group/Queen Mary University of London
pubs.organisational-group/Queen Mary University of London/Faculty of Medicine & Dentistry
pubs.organisational-group/Queen Mary University of London/Faculty of Medicine & Dentistry/William Harvey Research Institute
pubs.organisational-group/Queen Mary University of London/Faculty of Medicine & Dentistry/William Harvey Research Institute/Endocrinology
pubs.organisational-group/Queen Mary University of London/Faculty of Medicine & Dentistry/William Harvey Research Institute/REF William Harvey Research Institute
pubs.organisational-group/Queen Mary University of London/Faculty of Science & Engineering
pubs.organisational-group/Queen Mary University of London/Faculty of Science & Engineering/Engineering and Materials Science - Staff
pubs.publication-statusPublished
pubs.volume22


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