The DNA replication initiation protein DnaD recognises a specific strand of the Bacillus subtilis chromosome origin
dc.contributor.author | Winterhalter, C | |
dc.contributor.author | Pelliciari, S | |
dc.contributor.author | Stevens, D | |
dc.contributor.author | Fenyk, S | |
dc.contributor.author | Marchand, E | |
dc.contributor.author | Cronin, NB | |
dc.contributor.author | Soultanas, P | |
dc.contributor.author | Costa, TRD | |
dc.contributor.author | Ilangovan, A | |
dc.contributor.author | Murray, H | |
dc.date.accessioned | 2023-11-09T10:14:10Z | |
dc.date.available | 2023-04-04 | |
dc.date.available | 2023-11-09T10:14:10Z | |
dc.date.issued | 2023 | |
dc.identifier.issn | 0305-1048 | |
dc.identifier.uri | https://qmro.qmul.ac.uk/xmlui/handle/123456789/91807 | |
dc.description.abstract | Genome replication is a fundamental biological activity shared by all organisms. Chromosomal replication proceeds bidirectionally from origins, requiring the loading of two helicases, one for each replisome. However, the molecular mechanisms underpinning helicase loading at bacterial chromosome origins (oriC) are unclear. Here we investigated the essential DNA replication initiation protein DnaD in the model organism Bacillus subtilis. A set of DnaD residues required for ssDNA binding was identified, and photo-crosslinking revealed that this ssDNA binding region interacts preferentially with one strand of oriC. Biochemical and genetic data support the model that DnaD recognizes a new single-stranded DNA (ssDNA) motif located in oriC, the DnaD Recognition Element (DRE). Considered with single particle cryo-electron microscopy (cryo-EM) imaging of DnaD, we propose that the location of the DRE within oriC orchestrates strand-specific recruitment of helicase during DNA replication initiation. These findings significantly advance our mechanistic understanding of bidirectional replication from a bacterial chromosome origin. | en_US |
dc.format.extent | 4322 - 4340 | |
dc.publisher | Oxford University Press | en_US |
dc.relation.ispartof | NUCLEIC ACIDS RESEARCH | |
dc.rights | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. | |
dc.rights | Attribution 3.0 United States | * |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/us/ | * |
dc.title | The DNA replication initiation protein DnaD recognises a specific strand of the Bacillus subtilis chromosome origin | en_US |
dc.type | Article | en_US |
dc.rights.holder | © The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research. | |
dc.identifier.doi | 10.1093/nar/gkad277 | |
pubs.author-url | https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000974686700001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=612ae0d773dcbdba3046f6df545e9f6a | en_US |
pubs.issue | 9 | en_US |
pubs.notes | Not known | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 51 | en_US |
rioxxterms.funder | Default funder | en_US |
rioxxterms.identifier.project | Default project | en_US |
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Except where otherwise noted, this item's license is described as This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.