| dc.description.abstract | Protoporphyrin IX (PPIX) is a photosensitizer in the photodynamic therapy. However, its aqueous insolubility is always a challenge for extensive applications. In this work, a water-soluble derivative of PPIX were synthesized by esterification of PPIX and polyethylene glycol (PEG400), and the PPIX derivative was polymerized with hexamethylene diisocyanate to form a water-soluble polyurethane. The PPIX derivatives were characterized and the results showed that the polyurethane had the highest singlet oxygen generation efficiency among PPIX and its derivatives. The photodynamic antibacterial activity of PPIX derivatives were investigated on esch-erichia coli, staphylococcus aureus and methicillin-rcsistant staphylococcus aureus respectively. The results indicated that the polyurethane reached about 100% antibacterial rate of 40 μmol/L based on PPIX unit after 405 nm laser irradiation for 5 min. The PPIX derivatives showed high biocompatibility when co-cultured with human adipose-derived stem cells and liver cancer cells (HePG2) in the dark. With exposed to 405 nm or 635 nm laser for 5 min, about 90% and 50% HePG2 cells were killed by polyurethane, which was the best a-mong PPIX and its derivatives. Therefore, the polyurethane has the potential to be used as photodynamic therapy for both antibacterial and anticancer treatment. | en_US |
